Feline IL-8 ELISA Kit PicoKine®

Feline IL-8 ELISA Kit PicoKine® (96 Tests) . Quantitate Feline IL-8 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the IL8 gene. Interleukin-8 (IL8) is a member of the CXC chemokine family. IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. The genes for IL8 have been co-localized on a 335-kb genomic fragment.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 125 94 112 110 8.51 7.7% Sample 2 424 384 414 334 389 10.85 2.8% Sample 3 1336 1659 1311 1334 1410 51.18 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 125 94 112 110 8.51 7.7% Sample 1 110 125 94 112 110 8.51 7.7% Sample 2 424 384 414 334 389 10.85 2.8% Sample 2 424 384 414 334 389 10.85 2.8% Sample 3 1336 1659 1311 1334 1410 51.18 3.6% Sample 3 1336 1659 1311 1334 1410 51.18 3.6%

Synonyms

Interleukin-8; IL-8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor; MDNCF-a; GCP/IL-8 protein IV; IL8/NAP1 form I; Interleukin-8; (Ala-IL-8) 77; GCP/IL-8 protein II; IL-8 (1-77) ; IL8/NAP1 form II; MDNCF-b; IL-8 (5-77) ; IL-8 (6-77) ; (Ser-IL-8) 72; GCP/IL-8 protein I; IL8/NAP1 form III; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF-c; Neutrophil-activating factor; NAF; IL-8 (7-77) ; GCP/IL-8 protein V; IL8/NAP1 form IV; IL-8 (8-77) ; GCP/IL-8 protein VI; IL8/NAP1 form V; IL-8 (9-77) ; GCP/IL-8 protein III; IL8/NAP1 form VI; CXCL8; IL8

Gene Name

CXCL8

Gene ID

493836

UniProt

Q9XSX5

Reactivity

Feline

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Widely distributed in various tissues.

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

62.5-4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 424 1336 96 395 1051 Standard deviation 5.16 21.75 84.84 5.12 12.72 24.38 CV (%) 4.7% 5.1% 6.4% 5.3% 3.2% 2.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 424 1336 96 395 1051 Standard deviation 5.16 21.75 84.84 5.12 12.72 24.38 CV (%) 4.7% 5.1% 6.4% 5.3% 3.2% 2.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 110 424 1336 96 395 1051 Mean (pg/ml) 110 424 1336 96 395 1051 Standard deviation 5.16 21.75 84.84 5.12 12.72 24.38 Standard deviation 5.16 21.75 84.84 5.12 12.72 24.38 CV (%) 4.7% 5.1% 6.4% 5.3% 3.2% 2.3% CV (%) 4.7% 5.1% 6.4% 5.3% 3.2% 2.3%

Sensitivity

<10 pg/mL

Function

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8 (6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8 (5-77) has increased activity on neutrophil activation and IL-8 (7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8 (1-77), respectively. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

11165 MW

Specificity

Natural and recombinant feline IL-8

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-8

Subcellular Location

Secreted.

Sequence Similarities

Contains 1 HMG box DNA-binding domain.

Protein Name

Interleukin-8

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.003 0.037 0.08 0.187 0.375 0.815 1.592 2.397 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.003 0.037 0.08 0.187 0.375 0.815 1.592 2.397 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.003 0.037 0.08 0.187 0.375 0.815 1.592 2.397 O.D. 0.003 0.037 0.08 0.187 0.375 0.815 1.592 2.397