Feline IL-1 beta ELISA Kit PicoKine®

Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB) -regulated genes. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 41 54 48 50 1.06 2.1% Sample 2 230 125 182 144 170 8.46 5.0% Sample 3 765 549 815 601 683 51.87 7.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 41 54 48 50 1.06 2.1% Sample 1 55 41 54 48 50 1.06 2.1% Sample 2 230 125 182 144 170 8.46 5.0% Sample 2 230 125 182 144 170 8.46 5.0% Sample 3 765 549 815 601 683 51.87 7.6% Sample 3 765 549 815 601 683 51.87 7.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 230 765 59 138 819 Standard deviation 1.16 17.71 24.56 1.39/td> 7.65 19.25 CV (%) 2.1% 7.7% 3.2% 2.4% 5.5% 2.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 230 765 59 138 819 Standard deviation 1.16 17.71 24.56 1.39/td> 7.65 19.25 CV (%) 2.1% 7.7% 3.2% 2.4% 5.5% 2.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 230 765 59 138 819 Mean (pg/ml) 55 230 765 59 138 819 Standard deviation 1.16 17.71 24.56 1.39/td> 7.65 19.25 Standard deviation 1.16 17.71 24.56 1.39/td> 7.65 19.25 CV (%) 2.1% 7.7% 3.2% 2.4% 5.5% 2.4% CV (%) 2.1% 7.7% 3.2% 2.4% 5.5% 2.4%

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 O.D. 0.007 0.048 0.095 0.182 0.36 0.712 1.419 2.221 Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 O.D. 0.007 0.048 0.095 0.182 0.36 0.712 1.419 2.221 Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 O.D. 0.007 0.048 0.095 0.182 0.36 0.712 1.419 2.221 O.D. 0.007 0.048 0.095 0.182 0.36 0.712 1.419 2.221