Feline IL-10 ELISA Kit PicoKine®

Feline IL-10 ELISA Kit PicoKine® (96 Tests) . Quantitate Feline IL-10 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 216 173 173 215 194 10.76 5.5% Sample 2 877 822 939 949 897 26.10 2.9% Sample 3 2140 2834 3514 2729 2804 169.66 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 216 173 173 215 194 10.76 5.5% Sample 1 216 173 173 215 194 10.76 5.5% Sample 2 877 822 939 949 897 26.10 2.9% Sample 2 877 822 939 949 897 26.10 2.9% Sample 3 2140 2834 3514 2729 2804 169.66 6.1% Sample 3 2140 2834 3514 2729 2804 169.66 6.1%

Synonyms

Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10

Gene Name

IL10

Gene ID

493683

UniProt

P55029

Reactivity

Feline

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Widely expressed with highest levels in lymphoid tissues. .

Applications

ELISA

Field of Research

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

125.0-8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 216 877 2140 150 930 3500 Standard deviation 6.76 18.77 111.07 3.33/td> 50.69 148.40 CV (%) 3.1% 2.1% 5.2% 2.2% 5.5% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 216 877 2140 150 930 3500 Standard deviation 6.76 18.77 111.07 3.33/td> 50.69 148.40 CV (%) 3.1% 2.1% 5.2% 2.2% 5.5% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 216 877 2140 150 930 3500 Mean (pg/ml) 216 877 2140 150 930 3500 Standard deviation 6.76 18.77 111.07 3.33/td> 50.69 148.40 Standard deviation 6.76 18.77 111.07 3.33/td> 50.69 148.40 CV (%) 3.1% 2.1% 5.2% 2.2% 5.5% 4.2% CV (%) 3.1% 2.1% 5.2% 2.2% 5.5% 4.2%

Sensitivity

<15 pg/mL

Function

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20575 MW

Specificity

Natural and recombinant feline IL-10

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 10

Subcellular Location

Secreted.

Protein Name

Receptor tyrosine-protein kinase erbB-2

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.052 0.109 0.151 0.235 0.369 0.657 1.208 2.045 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.052 0.109 0.151 0.235 0.369 0.657 1.208 2.045 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.052 0.109 0.151 0.235 0.369 0.657 1.208 2.045 O.D. 0.052 0.109 0.151 0.235 0.369 0.657 1.208 2.045