Equine IL-4 ELISA Kit PicoKine®

IL4R (INTERLEUKIN 4 RECEPTOR) is a human gene that encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. The IL4R gene is localized to 16p12.1-p11.2 by in situ hybridization and Southern blot analysis of DNA from a panel of mouse-human somatic cell lines. IL4 plays a major role in immunoglobulin E (IgE) production. Its signal is conferred to effector cells through binding to the alpha chain of the IL4 receptor. IL4 interacts with IL4R with high affinity, leading to dimerization with either the common gamma chain, a component of receptors for a number of cytokines, to create a type I receptor, or with IL13RA1 to form a type II receptor.The IL4R gene contains 12 exons and IL4R is a complex of at least 2 components, one of which is a novel affinity converting subunit that is critical for cellular signal transduction. See also IL13RA1 and IL13RA2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 97 69 115 67 87 4.41 5.1% Sample 2 494 297 321 291 351 24.80 7.1% Sample 3 1985 1477 1479 1164 1526 51.89 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 97 69 115 67 87 4.41 5.1% Sample 1 97 69 115 67 87 4.41 5.1% Sample 2 494 297 321 291 351 24.80 7.1% Sample 2 494 297 321 291 351 24.80 7.1% Sample 3 1985 1477 1479 1164 1526 51.89 3.4% Sample 3 1985 1477 1479 1164 1526 51.89 3.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 97 494 1985 106 371 1684 Standard deviation 3.71 17.09 40.10 8.11/td> 27.16 121.75 CV (%) 3.8% 3.5% 2.0% 7.7% 7.3% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 97 494 1985 106 371 1684 Standard deviation 3.71 17.09 40.10 8.11/td> 27.16 121.75 CV (%) 3.8% 3.5% 2.0% 7.7% 7.3% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 97 494 1985 106 371 1684 Mean (pg/ml) 97 494 1985 106 371 1684 Standard deviation 3.71 17.09 40.10 8.11/td> 27.16 121.75 Standard deviation 3.71 17.09 40.10 8.11/td> 27.16 121.75 CV (%) 3.8% 3.5% 2.0% 7.7% 7.3% 7.2% CV (%) 3.8% 3.5% 2.0% 7.7% 7.3% 7.2%

Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells (By similarity) . Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST) . Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.037 0.11 0.165 0.267 0.461 0.924 1.741 2.412 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.037 0.11 0.165 0.267 0.461 0.924 1.741 2.412 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.037 0.11 0.165 0.267 0.461 0.924 1.741 2.412 O.D. 0.037 0.11 0.165 0.267 0.461 0.924 1.741 2.412