Equine IFN-gamma ELISA Kit PicoKine®

Equine IFN-gamma ELISA Kit PicoKine® (96 Tests) . Quantitate Equine IFN-gamma in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interferon gamma receptor 1 (IFNGR1), also known as CD119 (Cluster of Differentiation 119), is a protein that in humans is encoded by the IFNGR1 gene. This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 102 106 75 115 100 5.01 5.0% Sample 2 390 460 427 364 410 9.93 2.4% Sample 3 1926 1421 1696 1966 1752 92.17 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 102 106 75 115 100 5.01 5.0% Sample 1 102 106 75 115 100 5.01 5.0% Sample 2 390 460 427 364 410 9.93 2.4% Sample 2 390 460 427 364 410 9.93 2.4% Sample 3 1926 1421 1696 1966 1752 92.17 5.3% Sample 3 1926 1421 1696 1966 1752 92.17 5.3%

Synonyms

Interferon gamma receptor 1; IFN-gamma receptor 1; IFN-gamma-R1; CDw119; CD119; IFNGR1

Gene Name

IFNG

Gene ID

100034181

UniProt

P42160

Reactivity

Equine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Liver and small intestine. Also found in ovary, testis and kidney.

Applications

ELISA

Field of Research

Antiviral Signaling, Cytokines, Hematopoietic Progenitors, Host-Virus Interaction, Immune System Diseases, Immunology, Innate Immunity, Interferons, Interspecies Interaction, Lymphoid, Macrophage/Inflammation, Microbiology, Myeloid, Stem Cells, Surface Molecules, T Lymphocytic Lineage

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

62.5-4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 390 1926 65 435 1094 Standard deviation 7.12 30.58 135.59 2.54/td> 10.79 44.64 CV (%) 7.0% 7.8% 7.0% 3.9% 2.5% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 390 1926 65 435 1094 Standard deviation 7.12 30.58 135.59 2.54/td> 10.79 44.64 CV (%) 7.0% 7.8% 7.0% 3.9% 2.5% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 102 390 1926 65 435 1094 Mean (pg/ml) 102 390 1926 65 435 1094 Standard deviation 7.12 30.58 135.59 2.54/td> 10.79 44.64 Standard deviation 7.12 30.58 135.59 2.54/td> 10.79 44.64 CV (%) 7.0% 7.8% 7.0% 3.9% 2.5% 4.1% CV (%) 7.0% 7.8% 7.0% 3.9% 2.5% 4.1%

Sensitivity

<10 pg/mL

Function

Associates with IFNGR2 to form a receptor for the cytokine interferon gamma (IFNG) (PubMed:7615558, PubMed:2971451, PubMed:7617032, PubMed:10986460) . Ligand binding stimulates activation of the JAK/STAT signaling pathway (PubMed:7673114) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

19338 MW

Specificity

Natural and recombinant equine IFN-gamma

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interferon gamma receptor 1

Subcellular Location

Cell membrane ; Single-pass type I membrane protein .

Protein Name

Interferon gamma receptor 1

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.034 0.177 0.296 0.493 0.854 1.322 1.927 2.215 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.034 0.177 0.296 0.493 0.854 1.322 1.927 2.215 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.034 0.177 0.296 0.493 0.854 1.322 1.927 2.215 O.D. 0.034 0.177 0.296 0.493 0.854 1.322 1.927 2.215