Equine IFN-gamma ELISA Kit PicoKine®
Equine IFN-gamma ELISA Kit PicoKine® (96 Tests) . Quantitate Equine IFN-gamma in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Product Specifications
Background
Interferon gamma receptor 1 (IFNGR1), also known as CD119 (Cluster of Differentiation 119), is a protein that in humans is encoded by the IFNGR1 gene. This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 102 106 75 115 100 5.01 5.0% Sample 2 390 460 427 364 410 9.93 2.4% Sample 3 1926 1421 1696 1966 1752 92.17 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 102 106 75 115 100 5.01 5.0% Sample 1 102 106 75 115 100 5.01 5.0% Sample 2 390 460 427 364 410 9.93 2.4% Sample 2 390 460 427 364 410 9.93 2.4% Sample 3 1926 1421 1696 1966 1752 92.17 5.3% Sample 3 1926 1421 1696 1966 1752 92.17 5.3%
Synonyms
Interferon gamma receptor 1; IFN-gamma receptor 1; IFN-gamma-R1; CDw119; CD119; IFNGR1
Gene Name
IFNG
Gene ID
100034181
UniProt
P42160
Reactivity
Equine
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Capture Antibody
Monoclonal antibody from goat
Detection Antibody
Monoclonal antibody from goat
Tissue Specificity
Liver and small intestine. Also found in ovary, testis and kidney.
Applications
ELISA
Field of Research
Antiviral Signaling, Cytokines, Hematopoietic Progenitors, Host-Virus Interaction, Immune System Diseases, Immunology, Innate Immunity, Interferons, Interspecies Interaction, Lymphoid, Macrophage/Inflammation, Microbiology, Myeloid, Stem Cells, Surface Molecules, T Lymphocytic Lineage
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, serum, plasma (heparin, EDTA)
Detection Range
62.5-4,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 390 1926 65 435 1094 Standard deviation 7.12 30.58 135.59 2.54/td> 10.79 44.64 CV (%) 7.0% 7.8% 7.0% 3.9% 2.5% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 390 1926 65 435 1094 Standard deviation 7.12 30.58 135.59 2.54/td> 10.79 44.64 CV (%) 7.0% 7.8% 7.0% 3.9% 2.5% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 102 390 1926 65 435 1094 Mean (pg/ml) 102 390 1926 65 435 1094 Standard deviation 7.12 30.58 135.59 2.54/td> 10.79 44.64 Standard deviation 7.12 30.58 135.59 2.54/td> 10.79 44.64 CV (%) 7.0% 7.8% 7.0% 3.9% 2.5% 4.1% CV (%) 7.0% 7.8% 7.0% 3.9% 2.5% 4.1%
Sensitivity
<10 pg/mL
Function
Associates with IFNGR2 to form a receptor for the cytokine interferon gamma (IFNG) (PubMed:7615558, PubMed:2971451, PubMed:7617032, PubMed:10986460) . Ligand binding stimulates activation of the JAK/STAT signaling pathway (PubMed:7673114) . .
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular Weight
19338 MW
Specificity
Natural and recombinant equine IFN-gamma
Anticoagulant
Heparin or EDTA
Gene Name Synonym
Interferon gamma receptor 1
Subcellular Location
Cell membrane ; Single-pass type I membrane protein .
Protein Name
Interferon gamma receptor 1
Sample Data
Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.034 0.177 0.296 0.493 0.854 1.322 1.927 2.215 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.034 0.177 0.296 0.493 0.854 1.322 1.927 2.215 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.034 0.177 0.296 0.493 0.854 1.322 1.927 2.215 O.D. 0.034 0.177 0.296 0.493 0.854 1.322 1.927 2.215
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