Porcine IL-1ra ELISA Kit PicoKine®

Porcine IL-1ra ELISA Kit PicoKine® (96 Tests) . Quantitate Porcine IL-1ra in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 96 176 107 149 132 8.98 6.8% Sample 2 677 723 470 606 619 45.62 7.4% Sample 3 2162 1802 1915 2492 2093 147.96 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 96 176 107 149 132 8.98 6.8% Sample 1 96 176 107 149 132 8.98 6.8% Sample 2 677 723 470 606 619 45.62 7.4% Sample 2 677 723 470 606 619 45.62 7.4% Sample 3 2162 1802 1915 2492 2093 147.96 7.1% Sample 3 2162 1802 1915 2492 2093 147.96 7.1%

Synonyms

Interleukin-1 receptor antagonist protein; IL-1RN; IL-1ra; IRAP; ICIL-1RA; IL1 inhibitor; Anakinra; IL1RN; IL1F3, IL1RA

Gene Name

IL1RN

Gene ID

403660

UniProt

Q9BEH0

Reactivity

Porcine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

The intracellular form of IL1RN is predominantly expressed in epithelial cells.

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

93.8-6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 96 677 2162 116 558 2111 Standard deviation 3.48 46.10 121.50 3.21/td> 28.63 85.71 CV (%) 3.6% 6.8% 5.6% 2.8% 5.1% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 96 677 2162 116 558 2111 Standard deviation 3.48 46.10 121.50 3.21/td> 28.63 85.71 CV (%) 3.6% 6.8% 5.6% 2.8% 5.1% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 96 677 2162 116 558 2111 Mean (pg/ml) 96 677 2162 116 558 2111 Standard deviation 3.48 46.10 121.50 3.21/td> 28.63 85.71 Standard deviation 3.48 46.10 121.50 3.21/td> 28.63 85.71 CV (%) 3.6% 6.8% 5.6% 2.8% 5.1% 4.1% CV (%) 3.6% 6.8% 5.6% 2.8% 5.1% 4.1%

Sensitivity

<15 pg/mL

Function

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

19922 MW

Specificity

Natural and recombinant porcine IL-1ra

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-1 receptor antagonist protein

Subcellular Location

Isoform 1: Secreted.

Protein Name

Telomeric repeat-binding factor 2

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.056 0.103 0.152 0.24 0.43 0.716 1.289 2.222 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.056 0.103 0.152 0.24 0.43 0.716 1.289 2.222 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.056 0.103 0.152 0.24 0.43 0.716 1.289 2.222 O.D. 0.056 0.103 0.152 0.24 0.43 0.716 1.289 2.222