Porcine IL-1ra ELISA Kit PicoKine®

The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 96 176 107 149 132 8.98 6.8% Sample 2 677 723 470 606 619 45.62 7.4% Sample 3 2162 1802 1915 2492 2093 147.96 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 96 176 107 149 132 8.98 6.8% Sample 1 96 176 107 149 132 8.98 6.8% Sample 2 677 723 470 606 619 45.62 7.4% Sample 2 677 723 470 606 619 45.62 7.4% Sample 3 2162 1802 1915 2492 2093 147.96 7.1% Sample 3 2162 1802 1915 2492 2093 147.96 7.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 96 677 2162 116 558 2111 Standard deviation 3.48 46.10 121.50 3.21/td> 28.63 85.71 CV (%) 3.6% 6.8% 5.6% 2.8% 5.1% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 96 677 2162 116 558 2111 Standard deviation 3.48 46.10 121.50 3.21/td> 28.63 85.71 CV (%) 3.6% 6.8% 5.6% 2.8% 5.1% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 96 677 2162 116 558 2111 Mean (pg/ml) 96 677 2162 116 558 2111 Standard deviation 3.48 46.10 121.50 3.21/td> 28.63 85.71 Standard deviation 3.48 46.10 121.50 3.21/td> 28.63 85.71 CV (%) 3.6% 6.8% 5.6% 2.8% 5.1% 4.1% CV (%) 3.6% 6.8% 5.6% 2.8% 5.1% 4.1%

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.056 0.103 0.152 0.24 0.43 0.716 1.289 2.222 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.056 0.103 0.152 0.24 0.43 0.716 1.289 2.222 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.056 0.103 0.152 0.24 0.43 0.716 1.289 2.222 O.D. 0.056 0.103 0.152 0.24 0.43 0.716 1.289 2.222