Porcine GM-CSF ELISA Kit PicoKine®

Porcine GM-CSF Kit PicoKine® (96 Tests) . Quantitate Porcine GM-CSF in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cytokine receptor common subunit beta is a protein that in humans is encoded by the CSF2RB gene. It is mapped to 15 E1; 15 37.36 cM. The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 17 23 20 22 0.71 3.2% Sample 2 88 86 95 64 83 3.45 4.2% Sample 3 339 363 402 267 343 14.40 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 17 23 20 22 0.71 3.2% Sample 1 29 17 23 20 22 0.71 3.2% Sample 2 88 86 95 64 83 3.45 4.2% Sample 2 88 86 95 64 83 3.45 4.2% Sample 3 339 363 402 267 343 14.40 4.2% Sample 3 339 363 402 267 343 14.40 4.2%

Synonyms

Cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; CD131; Csf2rb; Aic2b; Csf2rb1; Il3rb1

Gene Name

CSF2

Gene ID

397208

UniProt

Q29118

Reactivity

Porcine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Broadly expressed with higher expression in heart.

Applications

ELISA

Field of Research

Auditory System, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Neurogenesis, Neurology Process, Neuroscience, Nuclear, Sensory System, Signaling Pathways, Stem Cells, Transcription

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

15.6-1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 88 339 20 108 401 Standard deviation 2.04 4.95 21.80 0.55 4.21 14.72 CV (%) 7.0% 5.6% 6.4% 2.7% 3.9% 3.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 88 339 20 108 401 Standard deviation 2.04 4.95 21.80 0.55 4.21 14.72 CV (%) 7.0% 5.6% 6.4% 2.7% 3.9% 3.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 88 339 20 108 401 Mean (pg/ml) 29 88 339 20 108 401 Standard deviation 2.04 4.95 21.80 0.55 4.21 14.72 Standard deviation 2.04 4.95 21.80 0.55 4.21 14.72 CV (%) 7.0% 5.6% 6.4% 2.7% 3.9% 3.7% CV (%) 7.0% 5.6% 6.4% 2.7% 3.9% 3.7%

Sensitivity

<2 pg/mL

Function

High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

16254 MW

Specificity

Natural and recombinant porcine GM-CSF

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)

Subcellular Location

Membrane. Single-pass type I membrane protein.

Protein Name

Cone-rod homeobox protein

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.093 0.156 0.294 0.567 0.93 1.576 2.156 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.093 0.156 0.294 0.567 0.93 1.576 2.156 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.093 0.156 0.294 0.567 0.93 1.576 2.156 O.D. 0.007 0.093 0.156 0.294 0.567 0.93 1.576 2.156