Porcine IL-1 alpha ELISA Kit PicoKine®

Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18kDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 164 170 160 164 8.61 5.3% Sample 2 431 562 574 479 512 21.28 4.2% Sample 3 2423 2572 2318 1733 2262 135.69 6.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 164 170 160 164 8.61 5.3% Sample 1 162 164 170 160 164 8.61 5.3% Sample 2 431 562 574 479 512 21.28 4.2% Sample 2 431 562 574 479 512 21.28 4.2% Sample 3 2423 2572 2318 1733 2262 135.69 6.0% Sample 3 2423 2572 2318 1733 2262 135.69 6.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 431 2423 107 514 1684 Standard deviation 11.15 10.17 175.18 3.09 38.60 125.29 CV (%) 6.9% 2.4% 7.2% 2.9% 7.5% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 431 2423 107 514 1684 Standard deviation 11.15 10.17 175.18 3.09 38.60 125.29 CV (%) 6.9% 2.4% 7.2% 2.9% 7.5% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 162 431 2423 107 514 1684 Mean (pg/ml) 162 431 2423 107 514 1684 Standard deviation 11.15 10.17 175.18 3.09 38.60 125.29 Standard deviation 11.15 10.17 175.18 3.09 38.60 125.29 CV (%) 6.9% 2.4% 7.2% 2.9% 7.5% 7.4% CV (%) 6.9% 2.4% 7.2% 2.9% 7.5% 7.4%

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.008 0.058 0.107 0.195 0.398 0.739 1.478 2.257 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.008 0.058 0.107 0.195 0.398 0.739 1.478 2.257 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.008 0.058 0.107 0.195 0.398 0.739 1.478 2.257 O.D. 0.008 0.058 0.107 0.195 0.398 0.739 1.478 2.257