Porcine IL-12/IL-23 p40 ELISA Kit PicoKine®

Subunit beta of interleukin 12 (IL12B), also known as interleukin 12B, or interleukin-12 subunit p40, is a subunit of human interleukin 12. It is mapped to 5q33.3. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 68 60 58 61 3.91 6.4% Sample 2 248 320 258 257 271 14.97 5.5% Sample 3 822 1477 775 1414 1122 28.61 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 68 60 58 61 3.91 6.4% Sample 1 59 68 60 58 61 3.91 6.4% Sample 2 248 320 258 257 271 14.97 5.5% Sample 2 248 320 258 257 271 14.97 5.5% Sample 3 822 1477 775 1414 1122 28.61 2.6% Sample 3 822 1477 775 1414 1122 28.61 2.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 248 822 88 333 1444 Standard deviation 4.57 17.36 18.66 4.75 25.17 91.84 CV (%) 7.7% 7.0% 2.3% 5.4% 7.6% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 248 822 88 333 1444 Standard deviation 4.57 17.36 18.66 4.75 25.17 91.84 CV (%) 7.7% 7.0% 2.3% 5.4% 7.6% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 59 248 822 88 333 1444 Mean (pg/ml) 59 248 822 88 333 1444 Standard deviation 4.57 17.36 18.66 4.75 25.17 91.84 Standard deviation 4.57 17.36 18.66 4.75 25.17 91.84 CV (%) 7.7% 7.0% 2.3% 5.4% 7.6% 6.4% CV (%) 7.7% 7.0% 2.3% 5.4% 7.6% 6.4%

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.064 0.168 0.257 0.38 0.671 1.033 1.644 2.226 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.064 0.168 0.257 0.38 0.671 1.033 1.644 2.226 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.064 0.168 0.257 0.38 0.671 1.033 1.644 2.226 O.D. 0.064 0.168 0.257 0.38 0.671 1.033 1.644 2.226