Porcine IL-4 ELISA Kit PicoKine®

Porcine IL-4 ELISA Kit PicoKine® (96 Tests) . Quantitate Porcine IL-4 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-4 (IL-4), also known as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 265 177 267 184 223 16.74 7.5% Sample 2 1082 1234 785 664 941 49.42 5.3% Sample 3 4599 3416 3520 4443 3995 182.15 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 265 177 267 184 223 16.74 7.5% Sample 1 265 177 267 184 223 16.74 7.5% Sample 2 1082 1234 785 664 941 49.42 5.3% Sample 2 1082 1234 785 664 941 49.42 5.3% Sample 3 4599 3416 3520 4443 3995 182.15 4.6% Sample 3 4599 3416 3520 4443 3995 182.15 4.6%

Synonyms

Interleukin-4; IL-4; B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; IL4

Gene Name

IL4

Gene ID

397225

UniProt

O77762

Reactivity

Porcine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

Applications

ELISA

Field of Research

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

156.0-10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 265 1082 4599 201 1024 3115 Standard deviation 19.80 63.41 195.0 15.84 81.51 109.34 CV (%) 7.5% 5.9% 4.2% 7.9% 8.0% 3.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 265 1082 4599 201 1024 3115 Standard deviation 19.80 63.41 195.0 15.84 81.51 109.34 CV (%) 7.5% 5.9% 4.2% 7.9% 8.0% 3.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 265 1082 4599 201 1024 3115 Mean (pg/ml) 265 1082 4599 201 1024 3115 Standard deviation 19.80 63.41 195.0 15.84 81.51 109.34 Standard deviation 19.80 63.41 195.0 15.84 81.51 109.34 CV (%) 7.5% 5.9% 4.2% 7.9% 8.0% 3.5% CV (%) 7.5% 5.9% 4.2% 7.9% 8.0% 3.5%

Sensitivity

<20 pg/mL

Function

Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727) . It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity) .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

15267 MW

Specificity

Natural and recombinant porcine IL-4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 4

Subcellular Location

Secreted.

Protein Name

Glyceraldehyde-3-phosphate dehydrogenase

Sample Data

Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.143 0.189 0.302 0.502 0.904 1.469 2.031 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.143 0.189 0.302 0.502 0.904 1.469 2.031 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.143 0.189 0.302 0.502 0.904 1.469 2.031 O.D. 0.051 0.143 0.189 0.302 0.502 0.904 1.469 2.031