Porcine IFN-gamma ELISA Kit PicoKine®

Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 98 100 83 112 98 5.44 5.5% Sample 2 460 260 479 416 404 17.40 4.3% Sample 3 1282 1344 1868 1646 1535 82.12 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 98 100 83 112 98 5.44 5.5% Sample 1 98 100 83 112 98 5.44 5.5% Sample 2 460 260 479 416 404 17.40 4.3% Sample 2 460 260 479 416 404 17.40 4.3% Sample 3 1282 1344 1868 1646 1535 82.12 5.4% Sample 3 1282 1344 1868 1646 1535 82.12 5.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 98 460 1282 109 336 1200 Standard deviation 3.80 28.75 53.46 5.86 20.03 29.76 CV (%) 3.9% 6.3% 4.2% 5.4% 6.0% 2.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 98 460 1282 109 336 1200 Standard deviation 3.80 28.75 53.46 5.86 20.03 29.76 CV (%) 3.9% 6.3% 4.2% 5.4% 6.0% 2.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 98 460 1282 109 336 1200 Mean (pg/ml) 98 460 1282 109 336 1200 Standard deviation 3.80 28.75 53.46 5.86 20.03 29.76 Standard deviation 3.80 28.75 53.46 5.86 20.03 29.76 CV (%) 3.9% 6.3% 4.2% 5.4% 6.0% 2.5% CV (%) 3.9% 6.3% 4.2% 5.4% 6.0% 2.5%

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.009 0.104 0.185 0.344 0.626 1.106 1.712 2.34 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.009 0.104 0.185 0.344 0.626 1.106 1.712 2.34 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.009 0.104 0.185 0.344 0.626 1.106 1.712 2.34 O.D. 0.009 0.104 0.185 0.344 0.626 1.106 1.712 2.34