Porcine IL-1 beta ELISA Kit PicoKine®

Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB) -regulated genes. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 55 65 63 61 3.31 5.4% Sample 2 161 299 187 289 234 14.34 6.1% Sample 3 1241 677 1159 935 1004 59.02 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 55 65 63 61 3.31 5.4% Sample 1 61 55 65 63 61 3.31 5.4% Sample 2 161 299 187 289 234 14.34 6.1% Sample 2 161 299 187 289 234 14.34 6.1% Sample 3 1241 677 1159 935 1004 59.02 5.9% Sample 3 1241 677 1159 935 1004 59.02 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 161 1244 59 170 758 Standard deviation 2.78 4.03 63.44 1.19 11.88 46.31 CV (%) 4.6% 2.5% 5.1% 2.0% 7.0% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 161 1244 59 170 758 Standard deviation 2.78 4.03 63.44 1.19 11.88 46.31 CV (%) 4.6% 2.5% 5.1% 2.0% 7.0% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 61 161 1244 59 170 758 Mean (pg/ml) 61 161 1244 59 170 758 Standard deviation 2.78 4.03 63.44 1.19 11.88 46.31 Standard deviation 2.78 4.03 63.44 1.19 11.88 46.31 CV (%) 4.6% 2.5% 5.1% 2.0% 7.0% 6.1% CV (%) 4.6% 2.5% 5.1% 2.0% 7.0% 6.1%

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.015 0.132 0.221 0.437 0.757 1.307 2.007 2.448 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.015 0.132 0.221 0.437 0.757 1.307 2.007 2.448 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.015 0.132 0.221 0.437 0.757 1.307 2.007 2.448 O.D. 0.015 0.132 0.221 0.437 0.757 1.307 2.007 2.448