Porcine TNF-alpha ELISA Kit PicoKine®

Porcine TNF-alpha ELISA Kit PicoKine® (96 Tests) . Quantitate Porcine TNF-alpha in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TNFα (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 28 27 44 36 1.96 5.4% Sample 2 143 133 149 151 144 9.60 6.7% Sample 3 567 701 609 389 567 26.68 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 28 27 44 36 1.96 5.4% Sample 1 46 28 27 44 36 1.96 5.4% Sample 2 143 133 149 151 144 9.60 6.7% Sample 2 143 133 149 151 144 9.60 6.7% Sample 3 567 701 609 389 567 26.68 4.7% Sample 3 567 701 609 389 567 26.68 4.7%

Synonyms

Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2

Gene Name

TNF

Gene ID

397086

UniProt

P23563

Reactivity

Porcine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus.

Applications

ELISA

Field of Research

Atherosclerosis, Cancer, Cardiovascular, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Metabolism, Signal Transduction, TNF Superfamily, Vascular Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

23.4-1,500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 143 567 28 109 477 Standard deviation 1.96 2.90 22.62 1.07 3.44 15.93 CV (%) 4.3% 2.0% 4.0% 3.8% 3.2% 3.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 143 567 28 109 477 Standard deviation 1.96 2.90 22.62 1.07 3.44 15.93 CV (%) 4.3% 2.0% 4.0% 3.8% 3.2% 3.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 46 143 567 28 109 477 Mean (pg/ml) 46 143 567 28 109 477 Standard deviation 1.96 2.90 22.62 1.07 3.44 15.93 Standard deviation 1.96 2.90 22.62 1.07 3.44 15.93 CV (%) 4.3% 2.0% 4.0% 3.8% 3.2% 3.3% CV (%) 4.3% 2.0% 4.0% 3.8% 3.2% 3.3%

Sensitivity

<5 pg/mL

Function

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

25254 MW

Specificity

Natural and recombinant porcine TNF-alpha

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor

Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Sequence Similarities

Belongs to the IL-15/IL-21 family.

Protein Name

Tumor necrosis factor

Sample Data

Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.032 0.11 0.163 0.285 0.544 0.973 1.748 2.487 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.032 0.11 0.163 0.285 0.544 0.973 1.748 2.487 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.032 0.11 0.163 0.285 0.544 0.973 1.748 2.487 O.D. 0.032 0.11 0.163 0.285 0.544 0.973 1.748 2.487