Canine CCL5/RANTES ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 5 (also CCL5) is a protein which in humans is encoded by the CCL5 gene. It is also known as RANTES. This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 31 31 42 35 1.35 3.9% Sample 2 174 156 136 118 146 4.04 2.8% Sample 3 674 605 639 495 603 47.29 7.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 31 31 42 35 1.35 3.9% Sample 1 36 31 31 42 35 1.35 3.9% Sample 2 174 156 136 118 146 4.04 2.8% Sample 2 174 156 136 118 146 4.04 2.8% Sample 3 674 605 639 495 603 47.29 7.8% Sample 3 674 605 639 495 603 47.29 7.8%

C-C motif chemokine 5; EoCP; Eosinophil chemotactic cytokine; SIS-delta; Small-inducible cytokine A5; T cell-specific protein P228; TCP228; T-cell-specific protein RANTES; RANTES (3-68) ; RANTES (4-68) ; CCL5; D17S136E, SCYA5

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 174 674 31 125 716 Standard deviation 1.97 7.85 34.31 1.93 9.13 14.68 CV (%) 5.5% 4.5% 5.1% 6.2% 7.3% 2.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 174 674 31 125 716 Standard deviation 1.97 7.85 34.31 1.93 9.13 14.68 CV (%) 5.5% 4.5% 5.1% 6.2% 7.3% 2.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 174 674 31 125 716 Mean (pg/ml) 36 174 674 31 125 716 Standard deviation 1.97 7.85 34.31 1.93 9.13 14.68 Standard deviation 1.97 7.85 34.31 1.93 9.13 14.68 CV (%) 5.5% 4.5% 5.1% 6.2% 7.3% 2.1% CV (%) 5.5% 4.5% 5.1% 6.2% 7.3% 2.1%

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) . The processed form RANTES (3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1- infection. The second processed form RANTES (4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES (1- 68) and RANTES (3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils (PubMed:16791620, PubMed:1380064, PubMed:8525373, PubMed:9516414, PubMed:15923218) . May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed:23979485) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.03 0.14 0.242 0.424 0.77 1.165 1.678 2.15 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.03 0.14 0.242 0.424 0.77 1.165 1.678 2.15 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.03 0.14 0.242 0.424 0.77 1.165 1.678 2.15 O.D. 0.03 0.14 0.242 0.424 0.77 1.165 1.678 2.15