Canine HGFR ELISA Kit PicoKine®

Hepatocyte growth factor receptor (HGF receptor) is a protein that in humans is encoded by the MET gene. Enables hepatocyte growth factor receptor activity. Involved in cell surface receptor signaling pathway; modulation of chemical synaptic transmission; and positive chemotaxis. Acts upstream of or within several processes, including hepatocyte growth factor receptor signaling pathway; regulation of cytokine production; and striated muscle cell differentiation. Located in membrane. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; genitourinary system; and limb.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 166 269 303 178 229 11.45 5.0% Sample 2 1046 741 841 849 869 49.03 5.6% Sample 3 4476 3425 4666 3288 3964 212.46 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 166 269 303 178 229 11.45 5.0% Sample 1 166 269 303 178 229 11.45 5.0% Sample 2 1046 741 841 849 869 49.03 5.6% Sample 2 1046 741 841 849 869 49.03 5.6% Sample 3 4476 3425 4666 3288 3964 212.46 5.4% Sample 3 4476 3425 4666 3288 3964 212.46 5.4%

Hepatocyte growth factor receptor; HGF receptor;2.7.10.1; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met; MET

Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. .

Cancer, Cancer Susceptibility, Epigenetics and Nuclear Signaling, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Proto-Oncogenes, Receptor Tyrosine Kinases, Signal Transduction, Transcription, Tyrosine Kinases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 166 1046 4476 275 765 3942 Standard deviation 13.10 36.61 237.68 14.96 43.45 285.40 CV (%) 7.9% 3.5% 5.3% 5.4% 5.7% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 166 1046 4476 275 765 3942 Standard deviation 13.10 36.61 237.68 14.96 43.45 285.40 CV (%) 7.9% 3.5% 5.3% 5.4% 5.7% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 166 1046 4476 275 765 3942 Mean (pg/ml) 166 1046 4476 275 765 3942 Standard deviation 13.10 36.61 237.68 14.96 43.45 285.40 Standard deviation 13.10 36.61 237.68 14.96 43.45 285.40 CV (%) 7.9% 3.5% 5.3% 5.4% 5.7% 7.2% CV (%) 7.9% 3.5% 5.3% 5.4% 5.7% 7.2%

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.111 0.201 0.395 0.741 1.167 1.749 2.013 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.111 0.201 0.395 0.741 1.167 1.749 2.013 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.111 0.201 0.395 0.741 1.167 1.749 2.013 O.D. 0.001 0.111 0.201 0.395 0.741 1.167 1.749 2.013