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Canine GM-CSF ELISA Kit PicoKine®

Canine GM-CSF ELISA Kit PicoKine® (96 Tests) . Quantitate Canine GM-CSF in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cytokine receptor common subunit beta is a protein that in humans is encoded by the CSF2RB gene. It is mapped to 15 E1; 15 37.36 cM. The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP) .

Anticoagulant

Heparin or EDTA

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 183 158 249 198 4.48 2.3% Sample 2 1096 654 786 715 813 58.03 7.1% Sample 3 4354 3698 2531 4783 3842 99.11 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 183 158 249 198 4.48 2.3% Sample 1 203 183 158 249 198 4.48 2.3% Sample 2 1096 654 786 715 813 58.03 7.1% Sample 2 1096 654 786 715 813 58.03 7.1% Sample 3 4354 3698 2531 4783 3842 99.11 2.6% Sample 3 4354 3698 2531 4783 3842 99.11 2.6%

Synonyms

Cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; CD131; Csf2rb; Aic2b; Csf2rb1; Il3rb1

Gene Name

CSF2

Gene ID

403923

UniProt

P48749

Reactivity

Canine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Broadly expressed with higher expression in heart.

Applications

ELISA

Field of Research

Auditory System, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Neurogenesis, Neurology Process, Neuroscience, Nuclear, Sensory System, Signaling Pathways, Stem Cells, Transcription

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

156.0-10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 1096 4354 310 1131 2853 Standard deviation 14.74 82.75 94.92 7.50 89.35 132.95 CV (%) 7.3% 7.6% 2.2% 2.4% 7.9% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 1096 4354 310 1131 2853 Standard deviation 14.74 82.75 94.92 7.50 89.35 132.95 CV (%) 7.3% 7.6% 2.2% 2.4% 7.9% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 203 1096 4354 310 1131 2853 Mean (pg/ml) 203 1096 4354 310 1131 2853 Standard deviation 14.74 82.75 94.92 7.50 89.35 132.95 Standard deviation 14.74 82.75 94.92 7.50 89.35 132.95 CV (%) 7.3% 7.6% 2.2% 2.4% 7.9% 4.7% CV (%) 7.3% 7.6% 2.2% 2.4% 7.9% 4.7%

Sensitivity

<20 pg/mL

Function

High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Product Datasheet

https://www.bosterbio.com/datasheet?sku=EK2342

Product MSDS

https://www.bosterbio.com/msds?sku=EK2342

Calculated Molecular Weight

16137 MW

Specificity

Natural and recombinant canine GM-CSF

Gene Name Synonym

Colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)

Subcellular Location

Membrane. Single-pass type I membrane protein.

Protein Name

Cone-rod homeobox protein

Sample Data

Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 O.D. 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005
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