Canine IL-2 ELISA Kit PicoKine®

IL2RG (INTERLEUKIN 2 RECEPTOR, GAMMA), also known as Common gamma chain or CD132, is a cytokine receptor sub-unit that is common to the receptor complexes for at least six different interleukin receptors:IL-2, IL-4, IL-7, IL-9, IL-15 and interleukin-21 receptor. IL2RG is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. By fluorescence in situ hybridization and PCR amplification of somatic cell hybrid DNAs, The IL2RG gene is mapped to Xq13.1. The IL2RG gene contains 8 exons and spans approximately 4.2 kb. And the gamma chain is an indispensable component of the functional IL2 receptor. Lymphocytes expressing the common gamma chain can form functional receptors for these cytokine proteins, which transmit signals from one cell to another and programs of cellular differentiation. The sharing of the gamma subunit by several receptors explains why humans and mice that lack IL2 entirely show milder symptoms than those with IL2RG deficiency.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 79 109 86 91 2.27 2.5% Sample 2 335 371 371 264 335 15.25 4.6% Sample 3 1051 1193 1355 1553 1288 79.08 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 79 109 86 91 2.27 2.5% Sample 1 90 79 109 86 91 2.27 2.5% Sample 2 335 371 371 264 335 15.25 4.6% Sample 2 335 371 371 264 335 15.25 4.6% Sample 3 1051 1193 1355 1553 1288 79.08 6.1% Sample 3 1051 1193 1355 1553 1288 79.08 6.1%

Interferon alpha/beta receptor 1; IFN-R-1; IFN-alpha/beta receptor 1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; CRF2-1; Type I interferon receptor 1; IFNAR1; IFNAR

IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R. .

Cancer, Cytokines, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Interferons, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Tumor Immunology

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 335 1051 115 357 1643 Standard deviation 6.63 16.38 22.49 3.42 16.42 68.18 CV (%) 7.4% 4.9% 2.1% 3.0% 4.6% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 335 1051 115 357 1643 Standard deviation 6.63 16.38 22.49 3.42 16.42 68.18 CV (%) 7.4% 4.9% 2.1% 3.0% 4.6% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 335 1051 115 357 1643 Mean (pg/ml) 90 335 1051 115 357 1643 Standard deviation 6.63 16.38 22.49 3.42 16.42 68.18 Standard deviation 6.63 16.38 22.49 3.42 16.42 68.18 CV (%) 7.4% 4.9% 2.1% 3.0% 4.6% 4.2% CV (%) 7.4% 4.9% 2.1% 3.0% 4.6% 4.2%

Component of the receptor for type I interferons, including interferons alpha, IFNB1 and IFNW1 (PubMed:2153461, PubMed:7665574, PubMed:10049744, PubMed:14532120, PubMed:15337770, PubMed:21854986) . Functions in general as heterodimer with IFNAR2 (PubMed:7665574, PubMed:10049744, PubMed:21854986) . Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta- subunits themselves (PubMed:7665574, PubMed:21854986) . Can form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Isoform 1: Cell membrane ; Single- pass type I membrane protein . Late endosome . Lysosome . Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. .

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.012 0.044 0.086 0.167 0.325 0.595 1.137 1.887 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.012 0.044 0.086 0.167 0.325 0.595 1.137 1.887 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.012 0.044 0.086 0.167 0.325 0.595 1.137 1.887 O.D. 0.012 0.044 0.086 0.167 0.325 0.595 1.137 1.887