Canine IL-1 beta ELISA Kit PicoKine®

Interleukin-1β (IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB) -regulated genes. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 129 131 182 124 142 7.87 5.6% Sample 2 451 654 416 425 487 17.37 3.6% Sample 3 1884 1757 1805 2511 1989 64.25 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 129 131 182 124 142 7.87 5.6% Sample 1 129 131 182 124 142 7.87 5.6% Sample 2 451 654 416 425 487 17.37 3.6% Sample 2 451 654 416 425 487 17.37 3.6% Sample 3 1884 1757 1805 2511 1989 64.25 3.2% Sample 3 1884 1757 1805 2511 1989 64.25 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 129 451 1884 162 494 1931 Standard deviation 6.90 27.01 72.16 5.38 38.88 56.58 CV (%) 5.4% 6.0% 3.8% 3.3% 7.9% 2.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 129 451 1884 162 494 1931 Standard deviation 6.90 27.01 72.16 5.38 38.88 56.58 CV (%) 5.4% 6.0% 3.8% 3.3% 7.9% 2.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 129 451 1884 162 494 1931 Mean (pg/ml) 129 451 1884 162 494 1931 Standard deviation 6.90 27.01 72.16 5.38 38.88 56.58 Standard deviation 6.90 27.01 72.16 5.38 38.88 56.58 CV (%) 5.4% 6.0% 3.8% 3.3% 7.9% 2.9% CV (%) 5.4% 6.0% 3.8% 3.3% 7.9% 2.9%

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Concentration (pg/ml) 0 7.8 15.6 31.2 62.4 125 250 500 O.D. 0.05 0.157 0.259 0.481 0.836 1.339 1.923 2.255 Concentration (pg/ml) 0 7.8 15.6 31.2 62.4 125 250 500 O.D. 0.05 0.157 0.259 0.481 0.836 1.339 1.923 2.255 Concentration (pg/ml) 0 7.8 15.6 31.2 62.4 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.2 62.4 125 250 500 O.D. 0.05 0.157 0.259 0.481 0.836 1.339 1.923 2.255 O.D. 0.05 0.157 0.259 0.481 0.836 1.339 1.923 2.255