Canine IL-4 ELISA Kit PicoKine®

Interleukin-4 (IL-4), also known as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 129 131 182 124 142 7.87 5.6% Sample 2 451 654 416 425 487 17.37 3.6% Sample 3 1884 1757 1805 2511 1989 64.25 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 129 131 182 124 142 7.87 5.6% Sample 1 129 131 182 124 142 7.87 5.6% Sample 2 451 654 416 425 487 17.37 3.6% Sample 2 451 654 416 425 487 17.37 3.6% Sample 3 1884 1757 1805 2511 1989 64.25 3.2% Sample 3 1884 1757 1805 2511 1989 64.25 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 129 451 1884 162 494 1931 Standard deviation 6.90 27.01 72.16 5.38 38.88 56.58 CV (%) 5.4% 6.0% 3.8% 3.3% 7.9% 2.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 129 451 1884 162 494 1931 Standard deviation 6.90 27.01 72.16 5.38 38.88 56.58 CV (%) 5.4% 6.0% 3.8% 3.3% 7.9% 2.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 129 451 1884 162 494 1931 Mean (pg/ml) 129 451 1884 162 494 1931 Standard deviation 6.90 27.01 72.16 5.38 38.88 56.58 Standard deviation 6.90 27.01 72.16 5.38 38.88 56.58 CV (%) 5.4% 6.0% 3.8% 3.3% 7.9% 2.9% CV (%) 5.4% 6.0% 3.8% 3.3% 7.9% 2.9%

Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727) . It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.01 0.049 0.118 0.218 0.442 0.795 1.289 1.882 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.01 0.049 0.118 0.218 0.442 0.795 1.289 1.882 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.01 0.049 0.118 0.218 0.442 0.795 1.289 1.882 O.D. 0.01 0.049 0.118 0.218 0.442 0.795 1.289 1.882