Canine IL-10 ELISA Kit PicoKine®

Canine IL-10 ELISA Kit PicoKine® (96 Tests) . Quantitate Canine IL-10 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 52 59 60 48 55 1.19 2.2% Sample 2 136 180 221 130 167 9.95 6.0% Sample 3 514 934 883 903 809 20.62 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 52 59 60 48 55 1.19 2.2% Sample 1 52 59 60 48 55 1.19 2.2% Sample 2 136 180 221 130 167 9.95 6.0% Sample 2 136 180 221 130 167 9.95 6.0% Sample 3 514 934 883 903 809 20.62 2.6% Sample 3 514 934 883 903 809 20.62 2.6%

Synonyms

Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10

Gene Name

IL10

Gene ID

403628

UniProt

P48411

Reactivity

Canine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Widely expressed with highest levels in lymphoid tissues. .

Applications

ELISA

Field of Research

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

31.2-2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 52 136 514 62 155 721 Standard deviation 3.84 7.75 23.28 4.74 7.41 15.21 CV (%) 7.4% 5.7% 4.5% 7.7% 4.8% 2.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 52 136 514 62 155 721 Standard deviation 3.84 7.75 23.28 4.74 7.41 15.21 CV (%) 7.4% 5.7% 4.5% 7.7% 4.8% 2.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 52 136 514 62 155 721 Mean (pg/ml) 52 136 514 62 155 721 Standard deviation 3.84 7.75 23.28 4.74 7.41 15.21 Standard deviation 3.84 7.75 23.28 4.74 7.41 15.21 CV (%) 7.4% 5.7% 4.5% 7.7% 4.8% 2.1% CV (%) 7.4% 5.7% 4.5% 7.7% 4.8% 2.1%

Sensitivity

<5 pg/mL

Function

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20906 MW

Specificity

Natural and recombinant canine IL-10

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 10

Subcellular Location

Secreted.

Protein Name

Receptor tyrosine-protein kinase erbB-2

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.107 0.189 0.379 0.668 1.08 1.624 2.217 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.107 0.189 0.379 0.668 1.08 1.624 2.217 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.107 0.189 0.379 0.668 1.08 1.624 2.217 O.D. 0.062 0.107 0.189 0.379 0.668 1.08 1.624 2.217