What is the catalog number for Canine IL-10 ELISA Kit PicoKine®?

The catalog number for Canine IL-10 ELISA Kit PicoKine® is EK2335.

What sizes are available for Canine IL-10 ELISA Kit PicoKine®?

Canine IL-10 ELISA Kit PicoKine® is available in the following size(s): 96 Well With Removable Strips.

Is Canine IL-10 ELISA Kit PicoKine® in stock?

Canine IL-10 ELISA Kit PicoKine® is currently made to order / not held in immediate stock. Contact us for lead time.

Where can I find the safety data sheet (SDS/MSDS) for Canine IL-10 ELISA Kit PicoKine®?

The safety data sheet for Canine IL-10 ELISA Kit PicoKine® is available here: https://www.bosterbio.com/msds?sku=EK2335.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

Canine IL-10 ELISA Kit PicoKine®

Canine IL-10 ELISA Kit PicoKine® (96 Tests) . Quantitate Canine IL-10 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 52 59 60 48 55 1.19 2.2% Sample 2 136 180 221 130 167 9.95 6.0% Sample 3 514 934 883 903 809 20.62 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 52 59 60 48 55 1.19 2.2% Sample 1 52 59 60 48 55 1.19 2.2% Sample 2 136 180 221 130 167 9.95 6.0% Sample 2 136 180 221 130 167 9.95 6.0% Sample 3 514 934 883 903 809 20.62 2.6% Sample 3 514 934 883 903 809 20.62 2.6%

Synonyms

Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10

Gene Name

IL10

Gene ID

403628

UniProt

P48411

Reactivity

Canine

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Widely expressed with highest levels in lymphoid tissues. .

Applications

ELISA

Field of Research

Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

31.2-2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 52 136 514 62 155 721 Standard deviation 3.84 7.75 23.28 4.74 7.41 15.21 CV (%) 7.4% 5.7% 4.5% 7.7% 4.8% 2.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 52 136 514 62 155 721 Standard deviation 3.84 7.75 23.28 4.74 7.41 15.21 CV (%) 7.4% 5.7% 4.5% 7.7% 4.8% 2.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 52 136 514 62 155 721 Mean (pg/ml) 52 136 514 62 155 721 Standard deviation 3.84 7.75 23.28 4.74 7.41 15.21 Standard deviation 3.84 7.75 23.28 4.74 7.41 15.21 CV (%) 7.4% 5.7% 4.5% 7.7% 4.8% 2.1% CV (%) 7.4% 5.7% 4.5% 7.7% 4.8% 2.1%

Sensitivity

<5 pg/mL

Function

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20906 MW

Specificity

Natural and recombinant canine IL-10

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 10

Subcellular Location

Secreted.

Protein Name

Receptor tyrosine-protein kinase erbB-2

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.107 0.189 0.379 0.668 1.08 1.624 2.217 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.107 0.189 0.379 0.668 1.08 1.624 2.217 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.107 0.189 0.379 0.668 1.08 1.624 2.217 O.D. 0.062 0.107 0.189 0.379 0.668 1.08 1.624 2.217