Mouse/Rat RGM-A ELISA Kit PicoKine®

Mouse/Rat RGM-A ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse/Rat RGM-A in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 106 108 156 98 117 4.98 4.3% Sample 2 577 593 385 518 518 28.56 5.5% Sample 3 1510 1548 2131 2465 1914 77.88 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 106 108 156 98 117 4.98 4.3% Sample 1 106 108 156 98 117 4.98 4.3% Sample 2 577 593 385 518 518 28.56 5.5% Sample 2 577 593 385 518 518 28.56 5.5% Sample 3 1510 1548 2131 2465 1914 77.88 4.1% Sample 3 1510 1548 2131 2465 1914 77.88 4.1%

Synonyms

Cyclic AMP-dependent transcription factor ATF-7; cAMP-dependent transcription factor ATF-7; Activating transcription factor 7; Transcription factor ATF-A; ATF7; ATFA

Gene Name

Rgma

Gene ID

244058

UniProt

Q6PCX7

Reactivity

Mouse/Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Expressed in heart, lung and skeletal muscle. Isoform 4 is expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta. .

Applications

ELISA

Field of Research

G Protein Signaling, Lipid Signaling, Signal Transduction, Signaling Pathway

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

78.1-5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 106 577 1510 80 473 1297 Standard deviation 7.59 20.83 79.43 6.38 33.25 69.15 CV (%) 7.2% 3.6% 5.3% 8.0% 7.0% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 106 577 1510 80 473 1297 Standard deviation 7.59 20.83 79.43 6.38 33.25 69.15 CV (%) 7.2% 3.6% 5.3% 8.0% 7.0% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 106 577 1510 80 473 1297 Mean (pg/ml) 106 577 1510 80 473 1297 Standard deviation 7.59 20.83 79.43 6.38 33.25 69.15 Standard deviation 7.59 20.83 79.43 6.38 33.25 69.15 CV (%) 7.2% 3.6% 5.3% 8.0% 7.0% 5.3% CV (%) 7.2% 3.6% 5.3% 8.0% 7.0% 5.3%

Sensitivity

<10 pg/mL

Function

Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]- 3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

49676 MW

Specificity

Natural and recombinant mouse/rat RGM-A

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cyclic AMP-dependent transcription factor ATF-7

Subcellular Location

Nucleus . Nucleus, nucleoplasm . Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Sample Data

Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.005 0.114 0.21 0.384 0.67 1.112 1.881 2.317 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.005 0.114 0.21 0.384 0.67 1.112 1.881 2.317 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.005 0.114 0.21 0.384 0.67 1.112 1.881 2.317 O.D. 0.005 0.114 0.21 0.384 0.67 1.112 1.881 2.317