Human VSIG1 ELISA Kit PicoKine®

Human VSIG1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human VSIG1 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

V-set and immunoglobulin domain containing 1 is a protein that in humans is encoded by the VSIG1 gene.The encoded protein contains multiple glycosylation sites at the N-terminal region, and multiple phosphorylation sites and glutamic acid/proline (EP) repeats at the C-terminal region. The gene is expressed in normal stomach and testis, as well as in gastric, esophageal and ovarian cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 61 38 44 51 3.04 6.0% Sample 2 162 209 172 134 169 6.35 3.8% Sample 3 926 887 512 974 825 62.52 7.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 61 38 44 51 3.04 6.0% Sample 1 61 61 38 44 51 3.04 6.0% Sample 2 162 209 172 134 169 6.35 3.8% Sample 2 162 209 172 134 169 6.35 3.8% Sample 3 926 887 512 974 825 62.52 7.6% Sample 3 926 887 512 974 825 62.52 7.6%

Synonyms

GPA34, dJ889N15.1, 1700062D20Rik

Gene Name

VSIG1

Gene ID

340547

UniProt

Q86XK7

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Detected only in stomach mucosa and testis, and to a much lesser level in pancreas (at protein level) . Detected in gastric cancers (31%), esophageal carcinomas (50%) and ovarian cancers (23%) .

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

31.2-2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 162 926 56 143 679 Standard deviation 3.54 12.21 44.91 3.43 9.11 21.05 CV (%) 5.8% 7.5% 4.9% 6.1% 6.4% 3.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 162 926 56 143 679 Standard deviation 3.54 12.21 44.91 3.43 9.11 21.05 CV (%) 5.8% 7.5% 4.9% 6.1% 6.4% 3.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 61 162 926 56 143 679 Mean (pg/ml) 61 162 926 56 143 679 Standard deviation 3.54 12.21 44.91 3.43 9.11 21.05 Standard deviation 3.54 12.21 44.91 3.43 9.11 21.05 CV (%) 5.8% 7.5% 4.9% 6.1% 6.4% 3.1% CV (%) 5.8% 7.5% 4.9% 6.1% 6.4% 3.1%

Sensitivity

<5 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

41811 MW

Specificity

Natural and recombinant human VSIG1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

V-set and immunoglobulin domain containing 1

Protein Name

V-set and immunoglobulin domain-containing protein 1

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.021 0.102 0.177 0.309 0.525 0.877 1.426 2.037 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.021 0.102 0.177 0.309 0.525 0.877 1.426 2.037 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.021 0.102 0.177 0.309 0.525 0.877 1.426 2.037 O.D. 0.021 0.102 0.177 0.309 0.525 0.877 1.426 2.037