Human VSIG8 ELISA Kit PicoKine®

V-set and immunoglobulin domain containing 8 is a protein that in humans is encoded by the VSIG8 gene. Enables RNA binding activity. Predicted to be located in membrane.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 48 40 53 70 53 3.13 5.9% Sample 2 241 284 194 158 219 9.47 4.3% Sample 3 831 879 759 969 860 36.96 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 48 40 53 70 53 3.13 5.9% Sample 1 48 40 53 70 53 3.13 5.9% Sample 2 241 284 194 158 219 9.47 4.3% Sample 2 241 284 194 158 219 9.47 4.3% Sample 3 831 879 759 969 860 36.96 4.3% Sample 3 831 879 759 969 860 36.96 4.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 48 241 831 71 259 1039 Standard deviation 2.51 10.24 19.28 3.42 15.54 72.31 CV (%) 5.2% 4.3% 2.3% 4.8% 6.0% 7.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 48 241 831 71 259 1039 Standard deviation 2.51 10.24 19.28 3.42 15.54 72.31 CV (%) 5.2% 4.3% 2.3% 4.8% 6.0% 7.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 48 241 831 71 259 1039 Mean (pg/ml) 48 241 831 71 259 1039 Standard deviation 2.51 10.24 19.28 3.42 15.54 72.31 Standard deviation 2.51 10.24 19.28 3.42 15.54 72.31 CV (%) 5.2% 4.3% 2.3% 4.8% 6.0% 7.0% CV (%) 5.2% 4.3% 2.3% 4.8% 6.0% 7.0%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.036 0.121 0.198 0.381 0.612 1.049 1.743 2.219 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.036 0.121 0.198 0.381 0.612 1.049 1.743 2.219 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.036 0.121 0.198 0.381 0.612 1.049 1.743 2.219 O.D. 0.036 0.121 0.198 0.381 0.612 1.049 1.743 2.219