Human RGM-A ELISA Kit PicoKine®

This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 176 171 188 180 179 10.62 5.9% Sample 2 891 962 892 736 870 19.15 2.2% Sample 3 3825 2087 2131 2557 2650 200.34 7.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 176 171 188 180 179 10.62 5.9% Sample 1 176 171 188 180 179 10.62 5.9% Sample 2 891 962 892 736 870 19.15 2.2% Sample 2 891 962 892 736 870 19.15 2.2% Sample 3 3825 2087 2131 2557 2650 200.34 7.6% Sample 3 3825 2087 2131 2557 2650 200.34 7.6%

Cyclic AMP-dependent transcription factor ATF-7; cAMP-dependent transcription factor ATF-7; Activating transcription factor 7; Transcription factor ATF-A; ATF7; ATFA

Expressed in heart, lung and skeletal muscle. Isoform 4 is expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 176 891 3825 148 616 2759 Standard deviation 12.88 66.02 118.58 10.03 15.95 156.44 CV (%) 7.3% 7.4% 3.1% 6.8% 2.6% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 176 891 3825 148 616 2759 Standard deviation 12.88 66.02 118.58 10.03 15.95 156.44 CV (%) 7.3% 7.4% 3.1% 6.8% 2.6% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 176 891 3825 148 616 2759 Mean (pg/ml) 176 891 3825 148 616 2759 Standard deviation 12.88 66.02 118.58 10.03 15.95 156.44 Standard deviation 12.88 66.02 118.58 10.03 15.95 156.44 CV (%) 7.3% 7.4% 3.1% 6.8% 2.6% 5.7% CV (%) 7.3% 7.4% 3.1% 6.8% 2.6% 5.7%

Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]- 3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Nucleus . Nucleus, nucleoplasm . Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery.

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.008 0.083 0.154 0.281 0.486 0.889 1.486 2.31 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.008 0.083 0.154 0.281 0.486 0.889 1.486 2.31 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.008 0.083 0.154 0.281 0.486 0.889 1.486 2.31 O.D. 0.008 0.083 0.154 0.281 0.486 0.889 1.486 2.31