Human Plasminogen ELISA Kit PicoKine®

PLAUR (PLASMINOGEN ACTIVATOR RECEPTOR, UROKINASE-TYPE), also known as UPAR or CD87, is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. PLAUR consists of three different domains of the Ly-6/uPAR/alpha-neurotoxin family. PLAUR is originally identified as a saturable binding site for urokinase on the cell surface. And the gene plays an important role in many normal as well as pathologic processes. The PLAUR gene is localized to 19q13.31. PLAUR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. PLAUR binds urokinase and thus restricts plasminogen activation to the immediate vicinity of the cell membrane. Thus it seems to be an important player in the regulation of this process. In human coronary artery vascular smooth muscle cells, UPA stimulates cell migration via a UPAR signaling complex containing TYK2 and phosphatidylinositol 3-kinase.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 90 116 152 123 6.31 5.2% Sample 2 603 488 433 573 524 30.51 5.8% Sample 3 2362 1434 1552 2292 1910 49.66 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 90 116 152 123 6.31 5.2% Sample 1 132 90 116 152 123 6.31 5.2% Sample 2 603 488 433 573 524 30.51 5.8% Sample 2 603 488 433 573 524 30.51 5.8% Sample 3 2362 1434 1552 2292 1910 49.66 2.6% Sample 3 2362 1434 1552 2292 1910 49.66 2.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 132 603 2362 96 603 1393 Standard deviation 7.68 32.74 170.77 5.62 15.86 57.67 CV (%) 5.8% 5.4% 7.2% 5.9% 2.6% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 132 603 2362 96 603 1393 Standard deviation 7.68 32.74 170.77 5.62 15.86 57.67 CV (%) 5.8% 5.4% 7.2% 5.9% 2.6% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 132 603 2362 96 603 1393 Mean (pg/ml) 132 603 2362 96 603 1393 Standard deviation 7.68 32.74 170.77 5.62 15.86 57.67 Standard deviation 7.68 32.74 170.77 5.62 15.86 57.67 CV (%) 5.8% 5.4% 7.2% 5.9% 2.6% 4.1% CV (%) 5.8% 5.4% 7.2% 5.9% 2.6% 4.1%

Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane . Cell projection, invadopodium membrane . Colocalized with FAP (seprase) preferentially at the cell surface of invadopodia membrane in a cytoskeleton-, integrin- and vitronectin-dependent manner. .

Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.009 0.077 0.156 0.257 0.448 0.761 1.188 2.012 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.009 0.077 0.156 0.257 0.448 0.761 1.188 2.012 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.009 0.077 0.156 0.257 0.448 0.761 1.188 2.012 O.D. 0.009 0.077 0.156 0.257 0.448 0.761 1.188 2.012