Human Neuropeptide Y/NPY ELISA Kit PicoKine®

Human Neuropeptide Y/NPY ELISA Kit PicoKine® (96 Tests) . Quantitate Human Neuropeptide Y/NPY in cell culture supernatants, tissue, serum, plasma (heparin, EDTA) . Sensitivity: 30pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 450 465 593 392 475 16.15 3.4% Sample 2 2022 1743 2055 2226 2012 121.09 6.0% Sample 3 6602 6303 8117 8708 7433 407.30 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 450 465 593 392 475 16.15 3.4% Sample 1 450 465 593 392 475 16.15 3.4% Sample 2 2022 1743 2055 2226 2012 121.09 6.0% Sample 2 2022 1743 2055 2226 2012 121.09 6.0% Sample 3 6602 6303 8117 8708 7433 407.30 5.5% Sample 3 6602 6303 8117 8708 7433 407.30 5.5%

Synonyms

Pro-neuropeptide Y; Neuropeptide Y; Neuropeptide tyrosine; NPY; C-flanking peptide of NPY; CPON; NPY

Gene Name

NPY

Gene ID

4852

UniProt

P01303

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.

Applications

ELISA

Field of Research

Biochemicals, Chemical Type, Neuropeptides, Neuroscience, Neurotransmitter, Obesity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, tissue, serum, plasma (heparin, EDTA)

Detection Range

312.0-10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 450 2022 6602 355 1400 7135 Standard deviation 18.23 75.83 270.68 12.60 91.84 487.32 CV (%) 4.1% 3.8% 4.1% 3.6% 6.6% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 450 2022 6602 355 1400 7135 Standard deviation 18.23 75.83 270.68 12.60 91.84 487.32 CV (%) 4.1% 3.8% 4.1% 3.6% 6.6% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 450 2022 6602 355 1400 7135 Mean (pg/ml) 450 2022 6602 355 1400 7135 Standard deviation 18.23 75.83 270.68 12.60 91.84 487.32 Standard deviation 18.23 75.83 270.68 12.60 91.84 487.32 CV (%) 4.1% 3.8% 4.1% 3.6% 6.6% 6.8% CV (%) 4.1% 3.8% 4.1% 3.6% 6.6% 6.8%

Sensitivity

<30 pg/mL

Function

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10851 MW

Specificity

Natural and recombinant human NPY

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Pro-neuropeptide Y

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the NPY family.

Protein Name

Pro-neuropeptide Y

Sample Data

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 O.D. 0.051 0.074 0.119 0.23 0.47 1.049 1.968 nan Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 O.D. 0.051 0.074 0.119 0.23 0.47 1.049 1.968 nan Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 O.D. 0.051 0.074 0.119 0.23 0.47 1.049 1.968 nan O.D. 0.051 0.074 0.119 0.23 0.47 1.049 1.968 nan