Human MyBPC3 ELISA Kit PicoKine®

Human MyBPC3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human MyBPC3 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The myosin-binding protein C, cardiac-type is a protein that in humans is encode by the MYBPC3 gene. MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 289 236 248 255 20.32 8.0% Sample 2 857 811 1169 706 886 57.04 6.4% Sample 3 2823 4400 4706 4814 4186 224.77 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 289 236 248 255 20.32 8.0% Sample 1 247 289 236 248 255 20.32 8.0% Sample 2 857 811 1169 706 886 57.04 6.4% Sample 2 857 811 1169 706 886 57.04 6.4% Sample 3 2823 4400 4706 4814 4186 224.77 5.4% Sample 3 2823 4400 4706 4814 4186 224.77 5.4%

Synonyms

Myosin-binding protein C, cardiac-type; Cardiac MyBP-C; C-protein, cardiac muscle isoform; MYBPC3

Gene Name

MYBPC3

Gene ID

4607

UniProt

Q14896

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Red blood cells.

Applications

ELISA

Field of Research

Cardiovascular, Contractility, Hypertrophy

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

156.0-10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 857 2823 268 1023 3733 Standard deviation 16.92 51.51 57.59 13.86 80.10 294.16 CV (%) 6.9% 6.0% 2.0% 5.2% 7.8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 857 2823 268 1023 3733 Standard deviation 16.92 51.51 57.59 13.86 80.10 294.16 CV (%) 6.9% 6.0% 2.0% 5.2% 7.8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 247 857 2823 268 1023 3733 Mean (pg/ml) 247 857 2823 268 1023 3733 Standard deviation 16.92 51.51 57.59 13.86 80.10 294.16 Standard deviation 16.92 51.51 57.59 13.86 80.10 294.16 CV (%) 6.9% 6.0% 2.0% 5.2% 7.8% 7.9% CV (%) 6.9% 6.0% 2.0% 5.2% 7.8% 7.9%

Sensitivity

<20 pg/mL

Function

Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

140762 MW

Specificity

Natural and recombinant human MyBPC3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Myosin binding protein C, cardiac

Sample Data

Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.066 0.124 0.26 0.496 0.907 1.388 2.025 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.066 0.124 0.26 0.496 0.907 1.388 2.025 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.066 0.124 0.26 0.496 0.907 1.388 2.025 O.D. 0.006 0.066 0.124 0.26 0.496 0.907 1.388 2.025