Human MBP ELISA Kit PicoKine®

Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. Human bikunin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa A1M protein (aa 20-203), and the C-terminal serine protease inhibitor bikunin (aa 206-352) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 98 132 164 186 145 8.56 5.9% Sample 2 707 749 521 431 602 25.46 4.2% Sample 3 2469 2315 1867 1792 2111 159.99 7.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 98 132 164 186 145 8.56 5.9% Sample 1 98 132 164 186 145 8.56 5.9% Sample 2 707 749 521 431 602 25.46 4.2% Sample 2 707 749 521 431 602 25.46 4.2% Sample 3 2469 2315 1867 1792 2111 159.99 7.6% Sample 3 2469 2315 1867 1792 2111 159.99 7.6%

Protein AMBP; Alpha-1-microglobulin; Protein HC; Alpha-1 microglycoprotein; Complex-forming glycoprotein heterogeneous in charge; Inter-alpha-trypsin inhibitor light chain; ITI-LC; Bikunin; EDC1; HI-30; Uronic-acid-rich protein; Trypstatin; AMBP; HCP; ITIL

Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha- trypsin inhibitor is present in plasma and urine.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 98 707 2469 182 592 2732 Standard deviation 7.70 38.89 140.98 10.70 16.16 153.81 CV (%) 7.9% 5.5% 5.7% 5.9% 2.7% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 98 707 2469 182 592 2732 Standard deviation 7.70 38.89 140.98 10.70 16.16 153.81 CV (%) 7.9% 5.5% 5.7% 5.9% 2.7% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 98 707 2469 182 592 2732 Mean (pg/ml) 98 707 2469 182 592 2732 Standard deviation 7.70 38.89 140.98 10.70 16.16 153.81 Standard deviation 7.70 38.89 140.98 10.70 16.16 153.81 CV (%) 7.9% 5.5% 5.7% 5.9% 2.7% 5.6% CV (%) 7.9% 5.5% 5.7% 5.9% 2.7% 5.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.03 0.09 0.155 0.286 0.52 0.931 1.412 1.982 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.03 0.09 0.155 0.286 0.52 0.931 1.412 1.982 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.03 0.09 0.155 0.286 0.52 0.931 1.412 1.982 O.D. 0.03 0.09 0.155 0.286 0.52 0.931 1.412 1.982