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Human LAIR2 ELISA Kit PicoKine®

Human LAIR2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human LAIR2 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Leukocyte-associated immunoglobulin-like receptor 2 is a protein that in humans is encoded by the LAIR2 gene. It was identified by its similarity to leukocyte-associated immunoglobulin-like receptor 1, a membrane-bound receptor that modulates innate immune response. The protein encoded by this locus is a soluble receptor that may play roles in both inhibition of collagen-induced platelet aggregation and vessel formation during placental implantation. This gene maps to a region of 19q13.4, termed the leukocyte receptor cluster, which contains 29 genes in the immunoglobulin superfamily. Alternatively spliced transcript variants have been described for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 12 14 9 13 12 0.87 7.3% Sample 2 56 48 34 42 45 2.60 5.8% Sample 3 230 211 203 143 197 4.19 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 12 14 9 13 12 0.87 7.3% Sample 1 12 14 9 13 12 0.87 7.3% Sample 2 56 48 34 42 45 2.60 5.8% Sample 2 56 48 34 42 45 2.60 5.8% Sample 3 230 211 203 143 197 4.19 2.1% Sample 3 230 211 203 143 197 4.19 2.1%

Synonyms

CD306

Gene Name

LAIR2

Gene ID

3904

UniProt

Q6ISS4

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

7.8-500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 12 56 230 15 35 222 Standard deviation 0.93 3.24 13.04 0.77 1.31 17.34 CV (%) 7.7% 5.8% 5.7% 5.1% 3.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 12 56 230 15 35 222 Standard deviation 0.93 3.24 13.04 0.77 1.31 17.34 CV (%) 7.7% 5.8% 5.7% 5.1% 3.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 12 56 230 15 35 222 Mean (pg/ml) 12 56 230 15 35 222 Standard deviation 0.93 3.24 13.04 0.77 1.31 17.34 Standard deviation 0.93 3.24 13.04 0.77 1.31 17.34 CV (%) 7.7% 5.8% 5.7% 5.1% 3.7% 7.8% CV (%) 7.7% 5.8% 5.7% 5.1% 3.7% 7.8%

Sensitivity

<2 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

16280 MW

Specificity

Natural and recombinant human LAIRR2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Leukocyte associated immunoglobulin like receptor 2

Protein Name

Leukocyte-associated immunoglobulin-like receptor 2

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.2 62.4 125 250 500 O.D. 0.05 0.099 0.171 0.319 0.602 1.034 1.63 2.057 Concentration (pg/ml) 0 7.8 15.6 31.2 62.4 125 250 500 O.D. 0.05 0.099 0.171 0.319 0.602 1.034 1.63 2.057 Concentration (pg/ml) 0 7.8 15.6 31.2 62.4 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.2 62.4 125 250 500 O.D. 0.05 0.099 0.171 0.319 0.602 1.034 1.63 2.057 O.D. 0.05 0.099 0.171 0.319 0.602 1.034 1.63 2.057
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