Human KLK15 ELISA Kit PicoKine®

Kallikrein-15 is a protein that in humans is encoded by the KLK15 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. In prostate cancer, this gene has increased expression, which indicates its possible use as a diagnostic or prognostic marker for prostate cancer. The gene contains multiple polyadenylation sites and alternative splicing results in multiple transcript variants encoding distinct isoforms.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 123 123 98 115 4.2 3.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 3 1548 1817 1980 1824 1792 52.87 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 123 123 98 115 4.2 3.7% Sample 1 114 123 123 98 115 4.2 3.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 3 1548 1817 1980 1824 1792 52.87 3.0% Sample 3 1548 1817 1980 1824 1792 52.87 3.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.043 0.11 0.171 0.274 0.515 0.915 1.601 2.341 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.043 0.11 0.171 0.274 0.515 0.915 1.601 2.341 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.043 0.11 0.171 0.274 0.515 0.915 1.601 2.341 O.D. 0.043 0.11 0.171 0.274 0.515 0.915 1.601 2.341