Human KLK15 ELISA Kit PicoKine®

Human KLK15 ELISA Kit PicoKine® (96 Tests) . Quantitate Human KLK15 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kallikrein-15 is a protein that in humans is encoded by the KLK15 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. In prostate cancer, this gene has increased expression, which indicates its possible use as a diagnostic or prognostic marker for prostate cancer. The gene contains multiple polyadenylation sites and alternative splicing results in multiple transcript variants encoding distinct isoforms.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 123 123 98 115 4.2 3.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 3 1548 1817 1980 1824 1792 52.87 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 123 123 98 115 4.2 3.7% Sample 1 114 123 123 98 115 4.2 3.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 3 1548 1817 1980 1824 1792 52.87 3.0% Sample 3 1548 1817 1980 1824 1792 52.87 3.0%

Synonyms

ACO, HSRNASPH

Gene Name

KLK15

Gene ID

55554

UniProt

Q9H2R5

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Highest expression in the thyroid gland. Also expressed in the prostate, salivary, and adrenal glands and in the colon testis and kidney.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

39.1-2,500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6%

Sensitivity

<10 pg/mL

Function

Protease whose physiological substrate is not yet known.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

28087 MW

Specificity

Natural and recombinant human KLK15

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Kallikrein related peptidase 15

Sequence Similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein Name

Kallikrein-15

Sample Data

Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.043 0.11 0.171 0.274 0.515 0.915 1.601 2.341 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.043 0.11 0.171 0.274 0.515 0.915 1.601 2.341 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.043 0.11 0.171 0.274 0.515 0.915 1.601 2.341 O.D. 0.043 0.11 0.171 0.274 0.515 0.915 1.601 2.341