Mouse Hip ELISA Kit PicoKine®

Mouse Hip ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Hip in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Huntingtin-interacting protein 1 also known as HIP-1 is a protein that in humans is encoded by the HIP1 gene. The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 123 123 98 115 4.2 3.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 3 1548 1817 1980 1824 1792 52.87 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 123 123 98 115 4.2 3.7% Sample 1 114 123 123 98 115 4.2 3.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 2 306 487 264 326 346 22.99 6.7% Sample 3 1548 1817 1980 1824 1792 52.87 3.0% Sample 3 1548 1817 1980 1824 1792 52.87 3.0%

Synonyms

T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a; CD8A; MAL

Gene Name

Hip1

Gene ID

3092

UniProt

Q8VD75

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues. .

Applications

ELISA

Field of Research

Neurodegenerative Disease, Neurology Process, Neuroscience

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

62.5-4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 1548 67 408 1745 Mean (pg/ml) 114 306 1548 67 408 1745 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 Standard deviation 5.76 7.41 68.73 1.34 25.13 98.42 CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6% CV (%) 4.8% 2.4% 4.4% 2.0% 6.2% 5.6%

Sensitivity

<10 pg/mL

Function

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

115202 MW

Specificity

Natural and recombinant mouse Hip

Anticoagulant

Heparin or EDTA

Gene Name Synonym

T-cell surface glycoprotein CD8 alpha chain

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.034 0.117 0.171 0.272 0.486 0.874 1.446 2.149 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.034 0.117 0.171 0.272 0.486 0.874 1.446 2.149 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.034 0.117 0.171 0.272 0.486 0.874 1.446 2.149 O.D. 0.034 0.117 0.171 0.272 0.486 0.874 1.446 2.149