Human Siglec-6/CD327 ELISA Kit PicoKine®

Human Siglec-6/CD327 ELISA Kit PicoKine® (96 Tests) . Quantitate Human Siglec-6/CD327 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Sialic acid-binding Ig-like lectin 6 is a protein that in humans is encoded by the SIGLEC6 gene. This gene encodes a member of the SIGLEC (sialic acid binding immunoglobulin-like lectin) family of proteins. The encoded transmembrane receptor binds sialyl-TN glycans and leptin. Placental expression of the encoded protein is upregulated in preeclampsia.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 181 273 268 227 17.28 7.6% Sample 2 1173 780 958 1123 1009 45.48 4.5% Sample 3 2962 4451 4562 4357 4083 157.60 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 181 273 268 227 17.28 7.6% Sample 1 185 181 273 268 227 17.28 7.6% Sample 2 1173 780 958 1123 1009 45.48 4.5% Sample 2 1173 780 958 1123 1009 45.48 4.5% Sample 3 2962 4451 4562 4357 4083 157.60 3.9% Sample 3 2962 4451 4562 4357 4083 157.60 3.9%

Synonyms

CD327, CD33L, OBBP1, CD33L1, CD33L2, CDW327

Gene Name

SIGLEC6

Gene ID

946

UniProt

O43699

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Expressed at high levels in placenta (cyto- and syncytiotrophoblastic cells) and at lower levels in spleen, peripheral blood leukocytes (predominantly B-cells) and small intestine.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

156.0-10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 1173 2962 213 954 4140 Standard deviation 4.88 81.76 186.31 13.16 27.67 290.63 CV (%) 2.6% 7.0% 6.3% 6.2% 2.9% 7.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 1173 2962 213 954 4140 Standard deviation 4.88 81.76 186.31 13.16 27.67 290.63 CV (%) 2.6% 7.0% 6.3% 6.2% 2.9% 7.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 185 1173 2962 213 954 4140 Mean (pg/ml) 185 1173 2962 213 954 4140 Standard deviation 4.88 81.76 186.31 13.16 27.67 290.63 Standard deviation 4.88 81.76 186.31 13.16 27.67 290.63 CV (%) 2.6% 7.0% 6.3% 6.2% 2.9% 7.0% CV (%) 2.6% 7.0% 6.3% 6.2% 2.9% 7.0%

Sensitivity

<20 pg/mL

Function

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

49913 MW

Specificity

Natural and recombinant human CD327

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Sialic acid binding Ig like lectin 6

Sequence Similarities

Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein Name

Sialic acid-binding Ig-like lectin 6

Sample Data

Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.093 0.152 0.259 0.466 0.877 1.428 2.13 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.093 0.152 0.259 0.466 0.877 1.428 2.13 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.093 0.152 0.259 0.466 0.877 1.428 2.13 O.D. 0.015 0.093 0.152 0.259 0.466 0.877 1.428 2.13