Human Siglec-2/CD22 ELISA Kit PicoKine®

CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. And CD22 is located within the band region q13.1 of chromosome 19.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 186 124 142 144 5.54 3.9% Sample 2 527 517 596 400 510 17.44 3.4% Sample 3 2678 2483 2654 1765 2395 58.2 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 186 124 142 144 5.54 3.9% Sample 1 124 186 124 142 144 5.54 3.9% Sample 2 527 517 596 400 510 17.44 3.4% Sample 2 527 517 596 400 510 17.44 3.4% Sample 3 2678 2483 2654 1765 2395 58.2 2.4% Sample 3 2678 2483 2654 1765 2395 58.2 2.4%

B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD22; CD22; SIGLEC2

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 527 2678 181 625 2097 Standard deviation 7.14 16.02 143.81 10.50 48.38 125.61 CV (%) 5.8% 3.0% 5.4% 5.8% 7.7% 6.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 527 2678 181 625 2097 Standard deviation 7.14 16.02 143.81 10.50 48.38 125.61 CV (%) 5.8% 3.0% 5.4% 5.8% 7.7% 6.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 124 527 2678 181 625 2097 Mean (pg/ml) 124 527 2678 181 625 2097 Standard deviation 7.14 16.02 143.81 10.50 48.38 125.61 Standard deviation 7.14 16.02 143.81 10.50 48.38 125.61 CV (%) 5.8% 3.0% 5.4% 5.8% 7.7% 6.0% CV (%) 5.8% 3.0% 5.4% 5.8% 7.7% 6.0%

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.006 0.131 0.244 0.434 0.757 1.382 1.943 2.234 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.006 0.131 0.244 0.434 0.757 1.382 1.943 2.234 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.006 0.131 0.244 0.434 0.757 1.382 1.943 2.234 O.D. 0.006 0.131 0.244 0.434 0.757 1.382 1.943 2.234