Human Siglec-9 ELISA Kit PicoKine®

Sialic acid-binding Ig-like lectin 9 is a protein that in humans is encoded by the SIGLEC9 gene. Predicted to enable monosaccharide binding activity and sialic acid binding activity. Predicted to be involved in cell adhesion. Predicted to act upstream of or within negative regulation of inflammatory response and negative regulation of phagocytosis, engulfment. Predicted to be located in external side of plasma membrane. Predicted to be active in plasma membrane.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 78 86 101 89 89 6.57 7.4% Sample 2 396 452 287 348 371 27.62 7.5% Sample 3 1461 1605 1396 1880 1586 56.44 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 78 86 101 89 89 6.57 7.4% Sample 1 78 86 101 89 89 6.57 7.4% Sample 2 396 452 287 348 371 27.62 7.5% Sample 2 396 452 287 348 371 27.62 7.5% Sample 3 1461 1605 1396 1880 1586 56.44 3.6% Sample 3 1461 1605 1396 1880 1586 56.44 3.6%

Expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils) . Found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 396 1461 69 478 1899 Standard deviation 4.63 14.89 74.80 5.21 36.23 146.41 CV (%) 5.9% 3.8% 5.1% 7.6% 7.6% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 396 1461 69 478 1899 Standard deviation 4.63 14.89 74.80 5.21 36.23 146.41 CV (%) 5.9% 3.8% 5.1% 7.6% 7.6% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 78 396 1461 69 478 1899 Mean (pg/ml) 78 396 1461 69 478 1899 Standard deviation 4.63 14.89 74.80 5.21 36.23 146.41 Standard deviation 4.63 14.89 74.80 5.21 36.23 146.41 CV (%) 5.9% 3.8% 5.1% 7.6% 7.6% 7.7% CV (%) 5.9% 3.8% 5.1% 7.6% 7.6% 7.7%

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.005 0.069 0.122 0.224 0.433 0.752 1.381 2.042 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.005 0.069 0.122 0.224 0.433 0.752 1.381 2.042 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.005 0.069 0.122 0.224 0.433 0.752 1.381 2.042 O.D. 0.005 0.069 0.122 0.224 0.433 0.752 1.381 2.042