Human Hyaluronan ELISA Kit PicoKine®

Cell migration-inducing and hyaluronan-binding protein (CEMIP), formerly known as KIAA1199, is a protein that in humans is encoded by the CEMIP gene. Enables several functions, including clathrin heavy chain binding activity; hyaluronic acid binding activity; and hyalurononglucosaminidase activity. Involved in several processes, including hyaluronan catabolic process; positive regulation of protein phosphorylation; and positive regulation of protein targeting to membrane. Located in several cellular components, including clathrin-coated endocytic vesicle; endoplasmic reticulum; and nucleus.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.06 6.3% Sample 2 3 2 2 3 3 0.11 4.4% Sample 3 24 20 29 26 25 0.69 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1 1 1 1 1 0.06 6.3% Sample 1 1 1 1 1 1 0.06 6.3% Sample 2 3 2 2 3 3 0.11 4.4% Sample 2 3 2 2 3 3 0.11 4.4% Sample 3 24 20 29 26 25 0.69 2.8% Sample 3 24 20 29 26 25 0.69 2.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 3 24 1 3 26 Standard deviation 0.06 0.06 1.02 0.05 0.12 0.52 CV (%) 6.0% 2.0% 4.2% 5.4% 4.0% 2.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1 3 24 1 3 26 Standard deviation 0.06 0.06 1.02 0.05 0.12 0.52 CV (%) 6.0% 2.0% 4.2% 5.4% 4.0% 2.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1 3 24 1 3 26 Mean (pg/ml) 1 3 24 1 3 26 Standard deviation 0.06 0.06 1.02 0.05 0.12 0.52 Standard deviation 0.06 0.06 1.02 0.05 0.12 0.52 CV (%) 6.0% 2.0% 4.2% 5.4% 4.0% 2.0% CV (%) 6.0% 2.0% 4.2% 5.4% 4.0% 2.0%

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity) . SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.4 0.74 1.11 3.33 10 30 90 O.D. 0.033 0.131 0.219 0.391 0.731 1.225 2.059 2.434 Concentration (ng/ml) 0 0.4 0.74 1.11 3.33 10 30 90 O.D. 0.033 0.131 0.219 0.391 0.731 1.225 2.059 2.434 Concentration (ng/ml) 0 0.4 0.74 1.11 3.33 10 30 90 Concentration (ng/ml) 0 0.4 0.74 1.11 3.33 10 30 90 O.D. 0.033 0.131 0.219 0.391 0.731 1.225 2.059 2.434 O.D. 0.033 0.131 0.219 0.391 0.731 1.225 2.059 2.434