Human Hepsin ELISA Kit PicoKine®

This gene encodes a type II transmembrane serine protease that may be involved in diverse cellular functions, including blood coagulation and the maintenance of cell morphology. Expression of the encoded protein is associated with the growth and progression of cancers, particularly prostate cancer. The protein is cleaved into a catalytic serine protease chain and a non-catalytic scavenger receptor cysteine-rich chain, which associate via a single disulfide bond. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 297 299 163 246 10.80 4.4% Sample 2 1132 906 943 782 941 50.14 5.3% Sample 3 3097 4339 3734 3883 3763 282.24 7.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 297 299 163 246 10.80 4.4% Sample 1 223 297 299 163 246 10.80 4.4% Sample 2 1132 906 943 782 941 50.14 5.3% Sample 2 1132 906 943 782 941 50.14 5.3% Sample 3 3097 4339 3734 3883 3763 282.24 7.5% Sample 3 3097 4339 3734 3883 3763 282.24 7.5%

HPN; TMPRSS1; Serine protease hepsin; EC 3.4.21.106; Transmembrane protease serine 1 [Cleaved into: Serine protease hepsin non-catalytic chain; Serine protease hepsin catalytic chain]

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 1132 3097 167 1008 4934 Standard deviation 13.38 22.75 132.55 5.46 35.48 372.52 CV (%) 6.0% 2.0% 4.3% 3.3% 3.5% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 1132 3097 167 1008 4934 Standard deviation 13.38 22.75 132.55 5.46 35.48 372.52 CV (%) 6.0% 2.0% 4.3% 3.3% 3.5% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 223 1132 3097 167 1008 4934 Mean (pg/ml) 223 1132 3097 167 1008 4934 Standard deviation 13.38 22.75 132.55 5.46 35.48 372.52 Standard deviation 13.38 22.75 132.55 5.46 35.48 372.52 CV (%) 6.0% 2.0% 4.3% 3.3% 3.5% 7.6% CV (%) 6.0% 2.0% 4.3% 3.3% 3.5% 7.6%

Serine protease that cleaves extracellular substrates, and contributes to the proteolytic processing of growth factors, such as HGF and MST1/HGFL; Plays a role in cell growth and maintenance of cell morphology; Plays a role in the proteolytic processing of ACE2; Mediates the proteolytic cleavage of urinary UMOD that is required for UMOD polymerization

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.132 0.22 0.392 0.742 1.231 2.06 2.435 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.132 0.22 0.392 0.742 1.231 2.06 2.435 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.034 0.132 0.22 0.392 0.742 1.231 2.06 2.435 O.D. 0.034 0.132 0.22 0.392 0.742 1.231 2.06 2.435