Human Glypican 4 ELISA Kit PicoKine®

Glypican-4 is a protein that in humans is encoded by the GPC4 gene. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 178 275 244 230 10.02 4.4% Sample 2 786 684 1162 695 832 62.55 7.5% Sample 3 4589 3143 3588 2859 3545 203.82 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 178 275 244 230 10.02 4.4% Sample 1 222 178 275 244 230 10.02 4.4% Sample 2 786 684 1162 695 832 62.55 7.5% Sample 2 786 684 1162 695 832 62.55 7.5% Sample 3 4589 3143 3588 2859 3545 203.82 5.8% Sample 3 4589 3143 3588 2859 3545 203.82 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 786 4589 249 939 4366 Standard deviation 16.85 62.64 124.36 11.16 60.28 130.98 CV (%) 7.6% 8.0% 2.7% 4.5% 6.4% 3.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 786 4589 249 939 4366 Standard deviation 16.85 62.64 124.36 11.16 60.28 130.98 CV (%) 7.6% 8.0% 2.7% 4.5% 6.4% 3.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 222 786 4589 249 939 4366 Mean (pg/ml) 222 786 4589 249 939 4366 Standard deviation 16.85 62.64 124.36 11.16 60.28 130.98 Standard deviation 16.85 62.64 124.36 11.16 60.28 130.98 CV (%) 7.6% 8.0% 2.7% 4.5% 6.4% 3.0% CV (%) 7.6% 8.0% 2.7% 4.5% 6.4% 3.0%

Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.109 0.217 0.447 0.816 1.281 1.897 2.337 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.109 0.217 0.447 0.816 1.281 1.897 2.337 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.109 0.217 0.447 0.816 1.281 1.897 2.337 O.D. 0.005 0.109 0.217 0.447 0.816 1.281 1.897 2.337