Human Glypican 4 ELISA Kit PicoKine®

Human Glypican 4 ELISA Kit PicoKine® (96 Tests) . Quantitate Human Glypican 4 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Glypican-4 is a protein that in humans is encoded by the GPC4 gene. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 178 275 244 230 10.02 4.4% Sample 2 786 684 1162 695 832 62.55 7.5% Sample 3 4589 3143 3588 2859 3545 203.82 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 178 275 244 230 10.02 4.4% Sample 1 222 178 275 244 230 10.02 4.4% Sample 2 786 684 1162 695 832 62.55 7.5% Sample 2 786 684 1162 695 832 62.55 7.5% Sample 3 4589 3143 3588 2859 3545 203.82 5.8% Sample 3 4589 3143 3588 2859 3545 203.82 5.8%

Synonyms

KPTS, K-glypican

Gene Name

GPC4

Gene ID

2239

UniProt

O75487

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

156.0-10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 786 4589 249 939 4366 Standard deviation 16.85 62.64 124.36 11.16 60.28 130.98 CV (%) 7.6% 8.0% 2.7% 4.5% 6.4% 3.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 786 4589 249 939 4366 Standard deviation 16.85 62.64 124.36 11.16 60.28 130.98 CV (%) 7.6% 8.0% 2.7% 4.5% 6.4% 3.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 222 786 4589 249 939 4366 Mean (pg/ml) 222 786 4589 249 939 4366 Standard deviation 16.85 62.64 124.36 11.16 60.28 130.98 Standard deviation 16.85 62.64 124.36 11.16 60.28 130.98 CV (%) 7.6% 8.0% 2.7% 4.5% 6.4% 3.0% CV (%) 7.6% 8.0% 2.7% 4.5% 6.4% 3.0%

Sensitivity

<20 pg/mL

Function

Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity) .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

62412 MW

Specificity

Natural and recombinant human Glypican 4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Glypican 4

Sequence Similarities

Belongs to the glypican family.

Protein Name

Glypican-4

Sample Data

Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.109 0.217 0.447 0.816 1.281 1.897 2.337 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.109 0.217 0.447 0.816 1.281 1.897 2.337 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.109 0.217 0.447 0.816 1.281 1.897 2.337 O.D. 0.005 0.109 0.217 0.447 0.816 1.281 1.897 2.337