Human GIT1 ELISA Kit PicoKine®

Human GIT1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human GIT1 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

ARF GTPase-activating protein GIT1 is an enzyme that in humans is encoded by the GIT1 gene. G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration. GIT-1 has also been implicated in neuronal functions including synapse formation and the pathology of Huntington disease. Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains. Htt interacts ly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 44 33 53 41 3.25 7.9% Sample 2 218 200 197 238 213 14.35 6.7% Sample 3 601 969 744 549 716 17.97 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 44 33 53 41 3.25 7.9% Sample 1 35 44 33 53 41 3.25 7.9% Sample 2 218 200 197 238 213 14.35 6.7% Sample 2 218 200 197 238 213 14.35 6.7% Sample 3 601 969 744 549 716 17.97 2.5% Sample 3 601 969 744 549 716 17.97 2.5%

Synonyms

DNA repair protein XRCC2; X-ray repair cross-complementing protein 2; Xrcc2

Gene Name

GIT1

Gene ID

Q9Y2X7

UniProt

Q9Y2X7

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Expressed at low level in somatic tissues and at high level in testis.

Applications

ELISA

Field of Research

Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

31.2-2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 218 601 49 144 808 Standard deviation 1.39 15.11 22.66 3.55 10.63 50.34 CV (%) 4.0% 6.9% 3.8% 7.3% 7.4% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 218 601 49 144 808 Standard deviation 1.39 15.11 22.66 3.55 10.63 50.34 CV (%) 4.0% 6.9% 3.8% 7.3% 7.4% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 218 601 49 144 808 Mean (pg/ml) 35 218 601 49 144 808 Standard deviation 1.39 15.11 22.66 3.55 10.63 50.34 Standard deviation 1.39 15.11 22.66 3.55 10.63 50.34 CV (%) 4.0% 6.9% 3.8% 7.3% 7.4% 6.2% CV (%) 4.0% 6.9% 3.8% 7.3% 7.4% 6.2%

Sensitivity

<5 pg/mL

Function

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

84341 MW

Specificity

Natural and recombinant human GIT1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

X-ray repair complementing defective repair in Chinese hamster cells 2

Subcellular Location

Nucleus.

Protein Name

DNA repair protein XRCC2

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 O.D. 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05