Human EDA2R/TNFRSF27/XEDAR ELISA Kit PicoKine®

Human EDA2R/TNFRSF27/XEDAR ELISA Kit PicoKine® (96 Tests) . Quantitate Human EDA2R/TNFRSF27/XEDAR in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tumor necrosis factor receptor superfamily member 27 is a protein that in humans is encoded by the EDA2R gene. This protein binds to the EDA-A2 isoform of ectodysplasin, which plays an important role in maintenance of hair and teeth. Alternatively spliced transcript variants encodes distinct protein isoforms.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 26 22 16 23 1.37 6.0% Sample 2 84 77 123 74 90 5.62 6.3% Sample 3 339 282 371 404 349 22.55 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 26 22 16 23 1.37 6.0% Sample 1 27 26 22 16 23 1.37 6.0% Sample 2 84 77 123 74 90 5.62 6.3% Sample 2 84 77 123 74 90 5.62 6.3% Sample 3 339 282 371 404 349 22.55 6.5% Sample 3 339 282 371 404 349 22.55 6.5%

Synonyms

XEDAR, EDAA2R, EDA-A2R, TNFRSF27

Gene Name

EDA2R

Gene ID

60401

UniProt

Q9HAV5

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

15.6-1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 84 339 25 79 269 Standard deviation 0.89 3.40 26.14 1.44 5.96 12.11 CV (%) 3.3% 4.1% 7.7% 5.8% 7.6% 4.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 84 339 25 79 269 Standard deviation 0.89 3.40 26.14 1.44 5.96 12.11 CV (%) 3.3% 4.1% 7.7% 5.8% 7.6% 4.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 27 84 339 25 79 269 Mean (pg/ml) 27 84 339 25 79 269 Standard deviation 0.89 3.40 26.14 1.44 5.96 12.11 Standard deviation 0.89 3.40 26.14 1.44 5.96 12.11 CV (%) 3.3% 4.1% 7.7% 5.8% 7.6% 4.5% CV (%) 3.3% 4.1% 7.7% 5.8% 7.6% 4.5%

Sensitivity

<2 pg/mL

Function

Receptor for EDA isoform A2, but not for EDA isoform A1. Mediates the activation of the NF-kappa-B and JNK pathways. Activation seems to be mediated by binding to TRAF3 and TRAF6.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

32759 MW

Specificity

Natural and recombinant human EDA2R

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Ectodysplasin A2 receptor

Protein Name

Tumor necrosis factor receptor superfamily member 27

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.057 0.148 0.211 0.358 0.571 1.009 1.803 2.393 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.057 0.148 0.211 0.358 0.571 1.009 1.803 2.393 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.057 0.148 0.211 0.358 0.571 1.009 1.803 2.393 O.D. 0.057 0.148 0.211 0.358 0.571 1.009 1.803 2.393