Human COL2A1 ELISA Kit PicoKine®

Human COL2A1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human COL2A1 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 187 298 211 187 221 10.68 4.8% Sample 2 1162 1101 1164 905 1083 21.98 2.0% Sample 3 3258 3486 3903 3808 3614 161.17 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 187 298 211 187 221 10.68 4.8% Sample 1 187 298 211 187 221 10.68 4.8% Sample 2 1162 1101 1164 905 1083 21.98 2.0% Sample 2 1162 1101 1164 905 1083 21.98 2.0% Sample 3 3258 3486 3903 3808 3614 161.17 4.5% Sample 3 3258 3486 3903 3808 3614 161.17 4.5%

Synonyms

Collagen alpha-1 (II) chain; Alpha-1 type II collagen; Collagen alpha-1 (II) chain; Chondrocalcin

Gene Name

COL2A1

Gene ID

1280

UniProt

P02458

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.

Applications

ELISA

Field of Research

Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells, Structures

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

156.0-10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 187 1162 3258 293 1198 4333 Standard deviation 12.66 45.67 185.38 11.90 87.33 346.21 CV (%) 6.8% 3.9% 5.7% 4.1% 7.3% 8.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 187 1162 3258 293 1198 4333 Standard deviation 12.66 45.67 185.38 11.90 87.33 346.21 CV (%) 6.8% 3.9% 5.7% 4.1% 7.3% 8.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 187 1162 3258 293 1198 4333 Mean (pg/ml) 187 1162 3258 293 1198 4333 Standard deviation 12.66 45.67 185.38 11.90 87.33 346.21 Standard deviation 12.66 45.67 185.38 11.90 87.33 346.21 CV (%) 6.8% 3.9% 5.7% 4.1% 7.3% 8.0% CV (%) 6.8% 3.9% 5.7% 4.1% 7.3% 8.0%

Sensitivity

<20 pg/mL

Function

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

141785 MW

Specificity

Natural and recombinant human COL2A1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Collagen, type II, alpha 1

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Sample Data

Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.071 0.136 0.292 0.524 0.917 1.632 2.258 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.071 0.136 0.292 0.524 0.917 1.632 2.258 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.071 0.136 0.292 0.524 0.917 1.632 2.258 O.D. 0.008 0.071 0.136 0.292 0.524 0.917 1.632 2.258