Human Cochlin ELISA Kit PicoKine®

Human Cochlin ELISA Kit PicoKine® (96 Tests) . QuantitateHuman Cochlin in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cochlin is a protein that in humans is encoded by the COCH gene.The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 37 39 34 42 3.22 7.8% Sample 2 212 238 180 203 208 9.83 4.7% Sample 3 572 780 689 964 751 51.76 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 37 39 34 42 3.22 7.8% Sample 1 56 37 39 34 42 3.22 7.8% Sample 2 212 238 180 203 208 9.83 4.7% Sample 2 212 238 180 203 208 9.83 4.7% Sample 3 572 780 689 964 751 51.76 6.9% Sample 3 572 780 689 964 751 51.76 6.9%

Synonyms

DFNA9, COCH5B2, DFNB110, COCH-5B2

Gene Name

COCH

Gene ID

1690

UniProt

O43405

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Expressed in inner ear structures; the cochlea and the vestibule.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

31.3-2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 212 572 60 196 845 Standard deviation 1.30 9.35 36.61 3.13 14.9 39.12 CV (%) 2.3% 4.4% 6.4% 5.2% 7.6% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 212 572 60 196 845 Standard deviation 1.30 9.35 36.61 3.13 14.9 39.12 CV (%) 2.3% 4.4% 6.4% 5.2% 7.6% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 56 212 572 60 196 845 Mean (pg/ml) 56 212 572 60 196 845 Standard deviation 1.30 9.35 36.61 3.13 14.9 39.12 Standard deviation 1.30 9.35 36.61 3.13 14.9 39.12 CV (%) 2.3% 4.4% 6.4% 5.2% 7.6% 4.6% CV (%) 2.3% 4.4% 6.4% 5.2% 7.6% 4.6%

Sensitivity

<5 pg/mL

Function

Plays a role in the control of cell shape and motility in the trabecular meshwork.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

59483 MW

Specificity

Natural and recombinant human Cochlin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cochlin

Protein Name

Cochlin

Sample Data

Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 O.D. 0.058 0.14 0.238 0.368 0.661 1.153 1.652 2.232 Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 O.D. 0.058 0.14 0.238 0.368 0.661 1.153 1.652 2.232 Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.3 62.5 125 250 500 1000 2000 O.D. 0.058 0.14 0.238 0.368 0.661 1.153 1.652 2.232 O.D. 0.058 0.14 0.238 0.368 0.661 1.153 1.652 2.232