Human CD96v2 ELISA Kit PicoKine®

CD96 (Cluster of Differentiation 96) or Tactile (T cell activation, increased late expression) is a protein that in humans is encoded by the CD96 gene. CD96 is a receptor protein which is expressed on T cells and NK cells and shares sequence similarity with CD226 (also known as DNAM-1) . The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein. The protein may play a role in the adhesion of activated T and NK cells to their target cells during the late phase of the immune response. It may also function in antigen presentation. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. CD96 is a transmembrane glycoprotein that has three extracellular immunoglobulin-like domains and is expressed by all resting human and mouse NK cells. CD96 main ligand is CD155. CD 96 has approximately 20% homology with CD226 and competed for binding to CD155 with CD226

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 141 144 192 152 5.45 3.6% Sample 2 817 989 754 739 825 36.87 4.5% Sample 3 3879 3470 3375 3792 3629 260.56 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 141 144 192 152 5.45 3.6% Sample 1 132 141 144 192 152 5.45 3.6% Sample 2 817 989 754 739 825 36.87 4.5% Sample 2 817 989 754 739 825 36.87 4.5% Sample 3 3879 3470 3375 3792 3629 260.56 7.2% Sample 3 3879 3470 3375 3792 3629 260.56 7.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 132 817 3879 141 534 3469 Standard deviation 4.55 52.12 189.3 5.94 17.03 162.70 CV (%) 3.5% 6.4% 4.9% 4.2% 3.2% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 132 817 3879 141 534 3469 Standard deviation 4.55 52.12 189.3 5.94 17.03 162.70 CV (%) 3.5% 6.4% 4.9% 4.2% 3.2% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 132 817 3879 141 534 3469 Mean (pg/ml) 132 817 3879 141 534 3469 Standard deviation 4.55 52.12 189.3 5.94 17.03 162.70 Standard deviation 4.55 52.12 189.3 5.94 17.03 162.70 CV (%) 3.5% 6.4% 4.9% 4.2% 3.2% 4.7% CV (%) 3.5% 6.4% 4.9% 4.2% 3.2% 4.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.008 0.092 0.166 0.327 0.596 1.022 1.679 2.236 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.008 0.092 0.166 0.327 0.596 1.022 1.679 2.236 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.008 0.092 0.166 0.327 0.596 1.022 1.679 2.236 O.D. 0.008 0.092 0.166 0.327 0.596 1.022 1.679 2.236