Human CD83 ELISA Kit PicoKine®

CD83 (Cluster of Differentiation 83) is a human protein encoded by the CD83 gene. The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 45 46 49 56 49 1.00 2.1% Sample 2 300 199 279 164 236 17.05 7.2% Sample 3 744 1082 854 858 885 43.08 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 45 46 49 56 49 1.00 2.1% Sample 1 45 46 49 56 49 1.00 2.1% Sample 2 300 199 279 164 236 17.05 7.2% Sample 2 300 199 279 164 236 17.05 7.2% Sample 3 744 1082 854 858 885 43.08 4.9% Sample 3 744 1082 854 858 885 43.08 4.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 45 300 744 51 235 834 Standard deviation 1.60 15.75 53.79 1.76 16.92 19.43 CV (%) 3.6% 5.3% 7.2% 3.5% 7.2% 2.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 45 300 744 51 235 834 Standard deviation 1.60 15.75 53.79 1.76 16.92 19.43 CV (%) 3.6% 5.3% 7.2% 3.5% 7.2% 2.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 45 300 744 51 235 834 Mean (pg/ml) 45 300 744 51 235 834 Standard deviation 1.60 15.75 53.79 1.76 16.92 19.43 Standard deviation 1.60 15.75 53.79 1.76 16.92 19.43 CV (%) 3.6% 5.3% 7.2% 3.5% 7.2% 2.3% CV (%) 3.6% 5.3% 7.2% 3.5% 7.2% 2.3%

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and ly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The ionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.005 0.04 0.079 0.173 0.344 0.612 1.218 2.075 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.005 0.04 0.079 0.173 0.344 0.612 1.218 2.075 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.005 0.04 0.079 0.173 0.344 0.612 1.218 2.075 O.D. 0.005 0.04 0.079 0.173 0.344 0.612 1.218 2.075