Human CD8 alpha ELISA Kit PicoKine®

Human CD8 alpha ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD8 alpha in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 21 29 29 24 26 0.61 2.4% Sample 2 110 72 98 84 91 3.44 3.8% Sample 3 440 440 425 364 417 27.91 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 21 29 29 24 26 0.61 2.4% Sample 1 21 29 29 24 26 0.61 2.4% Sample 2 110 72 98 84 91 3.44 3.8% Sample 2 110 72 98 84 91 3.44 3.8% Sample 3 440 440 425 364 417 27.91 6.7% Sample 3 440 440 425 364 417 27.91 6.7%

Synonyms

T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a; CD8A; MAL

Gene Name

CD8A

Gene ID

925

UniProt

P01732

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Brain.

Applications

ELISA

Field of Research

Adaptive Immunity, Immunology, T Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

15.6-1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 21 110 440 17 78 486 Standard deviation 0.52 7.35 11.04 0.57 2.39 15.7 CV (%) 2.5% 6.7% 2.5% 3.4% 3.1% 3.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 21 110 440 17 78 486 Standard deviation 0.52 7.35 11.04 0.57 2.39 15.7 CV (%) 2.5% 6.7% 2.5% 3.4% 3.1% 3.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 21 110 440 17 78 486 Mean (pg/ml) 21 110 440 17 78 486 Standard deviation 0.52 7.35 11.04 0.57 2.39 15.7 Standard deviation 0.52 7.35 11.04 0.57 2.39 15.7 CV (%) 2.5% 6.7% 2.5% 3.4% 3.1% 3.2% CV (%) 2.5% 6.7% 2.5% 3.4% 3.1% 3.2%

Sensitivity

<2 pg/mL

Function

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

25729 MW

Specificity

Natural and recombinant human CD8 alpha

Anticoagulant

Heparin or EDTA

Gene Name Synonym

T-cell surface glycoprotein CD8 alpha chain

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Protein Name

T-cell surface glycoprotein CD8 alpha chain

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.026 0.066 0.114 0.187 0.334 0.578 1.037 1.931 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.026 0.066 0.114 0.187 0.334 0.578 1.037 1.931 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.026 0.066 0.114 0.187 0.334 0.578 1.037 1.931 O.D. 0.026 0.066 0.114 0.187 0.334 0.578 1.037 1.931