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Mouse IL13RA2 ELISA Kit PicoKine®

Mouse IL13RA2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse IL13RA2 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-13 receptor subunit alpha-2 (IL-13Rα2), also known as CD213A2 (cluster of differentiation 213A2), is a membrane bound protein that in humans is encoded by the IL13RA2 gene. The protein encoded by this gene is closely related to Il13RA1, a subuint of the interleukin 13 receptor complex. This protein binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 77 60 51 71 65 2.05 3.2% Sample 2 203 160 214 273 213 4.55 2.1% Sample 3 787 1106 1007 1192 1023 68.34 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 77 60 51 71 65 2.05 3.2% Sample 1 77 60 51 71 65 2.05 3.2% Sample 2 203 160 214 273 213 4.55 2.1% Sample 2 203 160 214 273 213 4.55 2.1% Sample 3 787 1106 1007 1192 1023 68.34 6.7% Sample 3 787 1106 1007 1192 1023 68.34 6.7%

Synonyms

IL-13 receptor subunit alpha-2; IL-13R subunit alpha-2; IL-13R-alpha-2; IL-13RA2; Interleukin-13-binding protein; CD213a2

Gene Name

Il13ra2

Gene ID

16165

UniProt

O88786

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

39.0-2,500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77 203 787 41 255 888 Standard deviation 3.87 16.16 39.66 2.46 14.56 19.8 CV (%) 5.0% 8.0% 5.0% 6.0% 5.7% 2.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77 203 787 41 255 888 Standard deviation 3.87 16.16 39.66 2.46 14.56 19.8 CV (%) 5.0% 8.0% 5.0% 6.0% 5.7% 2.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 77 203 787 41 255 888 Mean (pg/ml) 77 203 787 41 255 888 Standard deviation 3.87 16.16 39.66 2.46 14.56 19.8 Standard deviation 3.87 16.16 39.66 2.46 14.56 19.8 CV (%) 5.0% 8.0% 5.0% 6.0% 5.7% 2.2% CV (%) 5.0% 8.0% 5.0% 6.0% 5.7% 2.2%

Sensitivity

<10 pg/mL

Function

Cell surface receptor that plays a role in the regulation of IL-13-mediated responses

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

44483 MW

Specificity

Natural and recombinant mouse IL13RA2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

IL13RA2

Subcellular Location

Cell membrane 1 publication; Single-pass type I membrane protein

Sequence Similarities

Belongs to the type I cytokine receptor family. Type 5 subfamily.

Protein Name

Interleukin-13 receptor subunit alpha-2

Sample Data

Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.02 0.082 0.145 0.251 0.462 0.748 1.201 2.053 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.02 0.082 0.145 0.251 0.462 0.748 1.201 2.053 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 Concentration (pg/ml) 0 39 78 156 312.5 625 1250 2500 O.D. 0.02 0.082 0.145 0.251 0.462 0.748 1.201 2.053 O.D. 0.02 0.082 0.145 0.251 0.462 0.748 1.201 2.053
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