Human Chymase ELISA Kit PicoKine®

Human Chymase ELISA Kit PicoKine® (96 Tests) . Quantitate Human Chymase in cell culture supernatants, serum, plasma (heparin) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chymase is a major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. Chymase is mapped to 14q12. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 156 109 119 127 5.79 4.6% Sample 2 610 433 733 546 581 31.99 5.5% Sample 3 2666 2285 2045 2561 2389 89.60 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 156 109 119 127 5.79 4.6% Sample 1 124 156 109 119 127 5.79 4.6% Sample 2 610 433 733 546 581 31.99 5.5% Sample 2 610 433 733 546 581 31.99 5.5% Sample 3 2666 2285 2045 2561 2389 89.60 3.8% Sample 3 2666 2285 2045 2561 2389 89.60 3.8%

Synonyms

Chymase;3.4.21.39; Alpha-chymase; Mast cell protease I; CMA1; CYH, CYM

Gene Name

CMA1

Gene ID

1215

UniProt

P23946

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. .

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Immunology, Innate Immunity, Macrophage/Inflammation, Signal Transduction, Vascular Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin)

Detection Range

93.8-6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 610 2666 119 449 2731 Standard deviation 3.30 29.83 152.76 5.08 35.38 59.81 CV (%) 2.7% 4.9% 5.7% 4.3% 7.9% 2.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 610 2666 119 449 2731 Standard deviation 3.30 29.83 152.76 5.08 35.38 59.81 CV (%) 2.7% 4.9% 5.7% 4.3% 7.9% 2.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 124 610 2666 119 449 2731 Mean (pg/ml) 124 610 2666 119 449 2731 Standard deviation 3.30 29.83 152.76 5.08 35.38 59.81 Standard deviation 3.30 29.83 152.76 5.08 35.38 59.81 CV (%) 2.7% 4.9% 5.7% 4.3% 7.9% 2.2% CV (%) 2.7% 4.9% 5.7% 4.3% 7.9% 2.2%

Sensitivity

<15 pg/mL

Function

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

27325 MW

Specificity

Natural and recombinant human Chymase

Anticoagulant

Heparin

Gene Name Synonym

Chymase

Subcellular Location

Secreted. Cytoplasmic granule. Mast cell granules.

Sequence Similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Protein Name

Chymase

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.073 0.119 0.167 0.265 0.447 0.798 1.357 2.079 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.073 0.119 0.167 0.265 0.447 0.798 1.357 2.079 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.073 0.119 0.167 0.265 0.447 0.798 1.357 2.079 O.D. 0.073 0.119 0.167 0.265 0.447 0.798 1.357 2.079