Human CCL26/Eotaxin-3 ELISA Kit PicoKine®

Eotaxin-3 is a small cytokine belonging to the CC chemokine family that is also called CCL26, Chemokine (C-C motif) ligand 26. Chemokines play pivotal roles in health and disease by controlling migration and tissue localization of specific types of cells expressing their cognate receptors. Eotaxin-3 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3. It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4. This gene for chemokine is located on human chromosome 7. Eotaxin-3 induced migration of eosinophils and basophils only at a 10-fold higher concentration than eotaxin.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 82 84 91 92 3.79 4.1% Sample 2 408 389 437 411 411 22.04 5.4% Sample 3 1376 1898 1803 1603 1670 115073 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 82 84 91 92 3.79 4.1% Sample 1 110 82 84 91 92 3.79 4.1% Sample 2 408 389 437 411 411 22.04 5.4% Sample 2 408 389 437 411 411 22.04 5.4% Sample 3 1376 1898 1803 1603 1670 115073 6.9% Sample 3 1376 1898 1803 1603 1670 115073 6.9%

C-C motif chemokine 26; CC chemokine IMAC; Eotaxin-3; Macrophage inflammatory protein 4-alpha; MIP-4-alpha; Small-inducible cytokine A26; Thymic stroma chemokine-1; TSC-1; CCL26; SCYA26; UNQ216/PRO242

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 408 1376 110 355 1001 Standard deviation 8.33 21.83 33.3 7.14 22.29 35.34 CV (%) 7.6% 5.4% 2.4% 6.5% 6.3% 3.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 408 1376 110 355 1001 Standard deviation 8.33 21.83 33.3 7.14 22.29 35.34 CV (%) 7.6% 5.4% 2.4% 6.5% 6.3% 3.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 110 408 1376 110 355 1001 Mean (pg/ml) 110 408 1376 110 355 1001 Standard deviation 8.33 21.83 33.3 7.14 22.29 35.34 Standard deviation 8.33 21.83 33.3 7.14 22.29 35.34 CV (%) 7.6% 5.4% 2.4% 6.5% 6.3% 3.5% CV (%) 7.6% 5.4% 2.4% 6.5% 6.3% 3.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.062 0.12 0.175 0.295 0.493 0.895 1.564 2.586 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.062 0.12 0.175 0.295 0.493 0.895 1.564 2.586 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.062 0.12 0.175 0.295 0.493 0.895 1.564 2.586 O.D. 0.062 0.12 0.175 0.295 0.493 0.895 1.564 2.586