Human CCL26/Eotaxin-3 ELISA Kit PicoKine®

Human CCL26/Eotaxin-3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CCL26/Eotaxin-3 in cell culture supernatants, serum, plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Eotaxin-3 is a small cytokine belonging to the CC chemokine family that is also called CCL26, Chemokine (C-C motif) ligand 26. Chemokines play pivotal roles in health and disease by controlling migration and tissue localization of specific types of cells expressing their cognate receptors. Eotaxin-3 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3. It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4. This gene for chemokine is located on human chromosome 7. Eotaxin-3 induced migration of eosinophils and basophils only at a 10-fold higher concentration than eotaxin.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 82 84 91 92 3.79 4.1% Sample 2 408 389 437 411 411 22.04 5.4% Sample 3 1376 1898 1803 1603 1670 115073 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 82 84 91 92 3.79 4.1% Sample 1 110 82 84 91 92 3.79 4.1% Sample 2 408 389 437 411 411 22.04 5.4% Sample 2 408 389 437 411 411 22.04 5.4% Sample 3 1376 1898 1803 1603 1670 115073 6.9% Sample 3 1376 1898 1803 1603 1670 115073 6.9%

Synonyms

C-C motif chemokine 26; CC chemokine IMAC; Eotaxin-3; Macrophage inflammatory protein 4-alpha; MIP-4-alpha; Small-inducible cytokine A26; Thymic stroma chemokine-1; TSC-1; CCL26; SCYA26; UNQ216/PRO242

Gene Name

CCL26

Gene ID

10344

UniProt

Q9Y258

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Capture Antibody

Monoclonal antibody from goat

Detection Antibody

Monoclonal antibody from goat

Tissue Specificity

Ubiquitously expressed at low levels in various tissues including heart and ovary.

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA)

Detection Range

62.5-4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 408 1376 110 355 1001 Standard deviation 8.33 21.83 33.3 7.14 22.29 35.34 CV (%) 7.6% 5.4% 2.4% 6.5% 6.3% 3.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 408 1376 110 355 1001 Standard deviation 8.33 21.83 33.3 7.14 22.29 35.34 CV (%) 7.6% 5.4% 2.4% 6.5% 6.3% 3.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 110 408 1376 110 355 1001 Mean (pg/ml) 110 408 1376 110 355 1001 Standard deviation 8.33 21.83 33.3 7.14 22.29 35.34 Standard deviation 8.33 21.83 33.3 7.14 22.29 35.34 CV (%) 7.6% 5.4% 2.4% 6.5% 6.3% 3.5% CV (%) 7.6% 5.4% 2.4% 6.5% 6.3% 3.5%

Sensitivity

<10 pg/mL

Function

Chemotactic for eosinophils and basophils. Binds to CCR3.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10648 MW

Specificity

Natural and recombinant human CCL26

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 26

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the intercrine beta (chemokine CC) family.

Protein Name

C-C motif chemokine 26

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.062 0.12 0.175 0.295 0.493 0.895 1.564 2.586 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.062 0.12 0.175 0.295 0.493 0.895 1.564 2.586 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.062 0.12 0.175 0.295 0.493 0.895 1.564 2.586 O.D. 0.062 0.12 0.175 0.295 0.493 0.895 1.564 2.586